Affinity Chromatography Poster

This poster was designed for the purpose of giving interested scienctists and students a comprehensive overview of the affinity chromatography method.

The poster covers many relevant subject and themes surrounding affinity chromatography, including:

• Different chromatography methods in comparison
   
• The design of an affinity bead and the chemistry behind it
   
• How to properly design a recombinant protein construct that can be used for affinity chromatography, including vector construction
   
• A detailed comparison between different commonly used affinity tags. (His-, GST-, MBP-, Strep-, Rho1D4-, FLAG-tag)
   
• The typical workflow of an affinity chromatography
   
• A list of the limitations of Affinity chromatography
   
• Sub-types of Affinity chromatography (Gravity flow, batch spon, FPLC, MagBeads)
   
• A list of potential subesquent studies