Affinity Chromatography Poster
Order number: Affinity-Poster
Description
This poster was designed for the purpose of giving interested scienctists and students a comprehensive overview of the affinity chromatography method. The poster covers many relevant subjects and themes surrounding affinity chromatography, including:
- Different chromatography methods in comparison
- The design of an affinity bead and the chemistry behind it
- How to properly design a recombinant protein construct that can be used for affinity chromatography, including vector construction
- A detailed comparison between different commonly used affinity tags. (His-, GST-, MBP-, Strep-, Rho1D4-, FLAG-tag)
- The typical workflow of an affinity chromatography
- A list of the limitations of Affinity chromatography
- Sub-types of Affinity chromatography (Gravity flow, Batch spin, FPLC, MagBeads)
- A list of potential subesquent studies