PureCube NTA MagBeads / magnetic beads were developed for IMAC based purification and enrichment methods. Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA magnetic beads uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. By providing our coupling protocols, feel free to create any NTA purification resin you desire. PureCube NTA MagBeads are also available XL sized for higher protein purifications of more viscous cell lysates or as NTA agarose resin. Furthermore PureCube NTA agarose can also be obtained pre-filled into FPLC cartridges / columns.
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Features
Usage
For coupling of metal ions for corresponding purification and / or enrichment purposes
Specifity
Varies
Binding capacity
Depends on the loaded ion
Purity
Depends on the loaded ion
Bead Ligand
NTA
Bead size
30 μm
Matrix
6% cross-linked agarose
Recommended FPLC flow rates
0.5-2 mL/min
Maximal FPLC flow rate
6 mL/min
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
