PureCube NTA Agarose
Order number: 31705
Description
PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 40 µm and thus great mechanical stability.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.
PureCube NTA resin is also available with a bead diameter of 100 µm or even XL sized (400 µm) for higher FPLC flow rates or as NTA magnetic beads. Furthermore, PureCube NTA agarose can also be obtained pre-packed into FPLC cartridges/columns.
However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.
PureCube NTA resin is also available with a bead diameter of 100 µm or even XL sized (400 µm) for higher FPLC flow rates or as NTA magnetic beads. Furthermore, PureCube NTA agarose can also be obtained pre-packed into FPLC cartridges/columns.
However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
- His-tag purification: Ni-NTA, Co-NTA, Zn-NTA, and Cu-NTA
- Phosphopeptide Enrichment: Al-NTA, Fe-NTA, Ti-NTA, and Zr-NTA. (Ti and Zr only as MagBeads)
| Feature | |
|---|---|
| Usage | For coupling of metal ions for corresponding purification and / or enrichment purposes |
| Specificity | Varies |
| Binding capacity | Depends on the loaded ion |
| Purity | Depends on the loaded ion |
| Bead Ligand | NTA |
| Bead size | 40 μm |
| Matrix | 7.5% cross-linked agarose |
| Filling quantity | Delivered as a 50 % suspension |
| Recommended FPLC flow rates | 0.5-2 ml/min |
| Maximal FPLC flow rate | 6 ml/min |
| pH stability | 2-14 |
| Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
Citations
| Coupled molecule / metal ion | Purified Substance | Year | Author |
|---|---|---|---|
| Gallium | Phages | 2019 | Schönberger N., Braun R., Matys S., Lederer F-L., Lehmann F., Flemming K., Pollmann K. |
| Gallium | Gallium binding Peptides | 2021 | Schönberger N. |
FAQ
Can I get the datasheet for the PureCube NTA resin?
Why is there a special protocol for the loading of nickel ions?
As Ni-NTA is the most commonly used NTA product, we have optimized this specific protocol.
Can I order a specific NTA product from you, even if it is not in your shop?
Yes, we offer a custom bead service for that.