PureCube Zr-NTA MagBeads
Order number: 31501-Zr
Description
PureCube Zr-NTA Magbeads / Magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of his tagged proteins as a secondary function. The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Zr-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding in a high degree of reproducibility. The phosphopeptide enrichment with Cube Biotech MagBeads can be automated as it has already been proven in this paper.
| Feature | |
|---|---|
| Usage |
|
| Specificity | Affinity to phosphorylated biomolecules< |
| Bead ligand | Zr-NTA |
| Bead size | 20-40 µm (30 µm average) |
| Matrix | Spherical magnetic agarose beads, cross-linked, 6% agarose |
| Filling quantity | 25% (v/v) suspension (20 µl suspension corresponds to 5 µl magnetic beads) |
| Compatible reagents | 1 mM DTT, 1 mM EDTA, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
| Metal ion capacity | >15 µeqv Zr4+ /ml |
| Stability | 2 years after shipping |
| Short Term Storage | in equilibration buffer (50% ACN, 50% MeOH, 0.01% acetic acid); 2-8°C |
| Long Term Storage | in 20% ethanol |
| Shipping | Room temperature |
Lab Results
Automatic Phosphoproteomics for high-throughput projects
Fe-NTA magnetic beads by Cube Biotech have been proven to increase the speed of a high throughput experiment drastically. Leutert et al. (2019) presented the application of PureCube Fe-NTA MagBeads in a procedure that they named R2-P2, which is short for Rapid-Robotic PhosphoProteomics. They used a KingFisherTM Flex for their robotic runs to fully automize the phosphopeptide enrichment process.
Fe-NTA magnetic beads by Cube Biotech have been proven to increase the speed of a high throughput experiment drastically. Leutert et al. (2019) presented the application of PureCube Fe-NTA MagBeads in a procedure that they named R2-P2, which is short for Rapid-Robotic PhosphoProteomics. They used a KingFisherTM Flex for their robotic runs to fully automize the phosphopeptide enrichment process.
Source: Leutert et al. (2019)
Superiority over other phosphopeptide enrichment methods
Leutert et al. compared three different types of IMAC beads (including our PureCube Fe-NTA) and TiO2 microspheres. As it can be seen in figure 2 our PureCube Fe-NTA magnetic beads presented themselves to be the best option for phosphopeptide enrichment. With our Fe-NTA MagBeads the most unique phosphopeptides (Fig. 2 A and C) were enriched with the highest efficiency (Figure 2 B).
Leutert et al. compared three different types of IMAC beads (including our PureCube Fe-NTA) and TiO2 microspheres. As it can be seen in figure 2 our PureCube Fe-NTA magnetic beads presented themselves to be the best option for phosphopeptide enrichment. With our Fe-NTA MagBeads the most unique phosphopeptides (Fig. 2 A and C) were enriched with the highest efficiency (Figure 2 B).
Source: Leutert et al. (2019)
The best product for the best prize
Analyzing and comparing products from various manufacturers and suppliers can often prove to be a laborious task. The presence of varying concentrations and volumes across different suppliers can lead to confusion when trying to identify the optimal cost-benefit ratio. Recognizing these challenges, Cube Biotech has undertaken the effort to compile a comprehensive overview of prices for the frequently utilized phosphopeptide enrichment products (see Fig. 2 C). Our intention is to provide researchers with a clearer understanding of the prevailing pricing landscape in the market, facilitating informed decision-making.
Analyzing and comparing products from various manufacturers and suppliers can often prove to be a laborious task. The presence of varying concentrations and volumes across different suppliers can lead to confusion when trying to identify the optimal cost-benefit ratio. Recognizing these challenges, Cube Biotech has undertaken the effort to compile a comprehensive overview of prices for the frequently utilized phosphopeptide enrichment products (see Fig. 2 C). Our intention is to provide researchers with a clearer understanding of the prevailing pricing landscape in the market, facilitating informed decision-making.
FAQ
Can I get the datasheet for the Zr-NTA MagBeads?
What can I do with Zr-NTA beads?
They are meant to be used as the matrix to bind and thus enrich phophopeptides.
How does the phosphopeptide enrichment process work?
Can I enrich all phosphopeptides using Zr-NTA?
No, this is not possible. Not all phosphopeptides bind to Zr-NTA beads. As shown in figure 2 multiples bead types must be used to cover all phosphopeptides off a cell. Because of that we also offer Ti- Fe- and Al-NTA beads.