AChR T. Californica
Order number: 28601
Description
Our AChR is directly purified from the acetylcholine receptor of Torpedo californica. It is the best example of how customized protein purification beads can be used to isolate a protein without any epitope tags. The protein was purified through an agarose resin coupled to neurotoxin-3 from king cobras, a natural ligand of AChR.
Datasheets
| Feature | |
|---|---|
| Alternative names | Acetylcholine receptor subunit alpha, beta, gamma and delta |
| UniProt Number | |
| Protein class | Ion channel, Ligand-gated ion channel, Receptor |
| Organism | Torpedo californica (electric ray) |
| Sequence, Alpha subunit One-Letter Code | MILCSYWHV GLVLLLFSCC GLVLGSEHET RLVANLLENY NKVIRPVEHH THFVDITVGL QLIQLISVDE VNQIVETNVR LRQQWIDVRL RWNPADYGGI KKIRLPSDDV WLPDLVLYNN ADGDFAIVHM TKLLLDYTGK IMWTPPAIFK SYCEIIVTHF PFDQQNCTMK LGIWTYDGTK VSISPESDRP DLSTFMESGE WVMKDYRGWK HWVYYTCCPD TPYLDITYHF IMQRIPLYFV VNVIIPCLLF SFLTGLVFYL PTDSGEKMTL SISVLLSLTV FLLVIVELIP STSSAVPLIG KYMLFTMIFV ISSIIITVVV INTHHRSPST HTMPQWVRKI FIDTIPNVMF FSTMKRASKE KQENKIFADD IDISDISGKQ VTGEVIFQTP LIKNPDVKSA IEGVKYIAEH MKSDEESSNA AEEWKYVAMV IDHILLCVFM LICIIGTVSV FAGRLIELSQ EG |
| Sequence, Beta subunit One-Letter Code | MENVRRMALG LVVMMALALS GVGASVMEDT LLSVLFETYN PKVRPAQTVG DKVTVRVGLT LTNLLILNEK IEEMTTNVFL NLAWTDYRLQ WDPAAYEGIK DLRIPSSDVW QPDIVLMNNN DGSFEITLHV NVLVQHTGAV SWQPSAIYRS SCTIKVMYFP FDWQNCTMVF KSYTYDTSEV TLQHALDAKG EREVKEIVIN KDAFTENGQW SIEHKPSRKN WRSDDPSYED VTFYLIIQRK PLFYIVYTII PCILISILAI LVFYLPPDAG EKMSLSISAL LAVTVFLLLL ADKVPETSLS VPIIIRYLMF IMILVAFSVI LSVVVLNLHH RSPNTHTMPN WIRQIFIETL PPFLWIQRPV TTPSPDSKPT IISRANDEYF IRKPAGDFVC PVDNARVAVQ PERLFSEMKW HLNGLTQPVT LPQDLKEAVE AIKYIAEQLE SASEFDDLKK DWQYVAMVAD RLFLYVFFVI CSIGTFSIFL DASHNVPPDN PFA |
| Sequence, Gamma subunit One-Letter Code | MVLTLLLIIC LALEVRSENE EGRLIEKLLG DYDKRIIPAK TLDHIIDVTL KLTLTNLISL NEKEEALTTN VWIEIQWNDY RLSWNTSEYE GIDLVRIPSE LLWLPDVVLE NNVDGQFEVA YYANVLVYND GSMYWLPPAI YRSTCPIAVT YFPFDWQNCS LVFRSQTYNA HEVNLQLSAE EGEAVEWIHI DPEDFTENGE WTIRHRPAKK NYNWQLTKDD TDFQEIIFFL IIQRKPLFYI INIIAPCVLI SSLVVLVYFL PAQAGGQKCT LSISVLLAQT IFLFLIAQKV PETSLNVPLI GKYLIFVMFV SMLIVMNCVI VLNVSLRTPN THSLSEKIKH LFLGFLPKYL GMQLEPSEET PEKPQPRRRS SFGIMIKAEE YILKKPRSEL MFEEQKDRHG LKRVNKMTSD IDIGTTVDLY KDLANFAPEI KSCVEACNFI AKSTKEQNDS GSENENWVLI GKVIDKACFW IALLLFSIGT LAIFLTGHFN QVPEFPFPGD PRKYVP |
| Sequence, Delta subunit One-Letter Code | MGNIHFVYLLISCLYYSGCS GVNEEERLIN DLLIVNKYNK HVRPVKHNNE VVNIALSLTL SNLISLKETD ETLTSNVWMD HAWYDHRLTW NASEYSDISI LRLPPELVWI PDIVLQNNND GQYHVAYFCN VLVRPNGYVT WLPPAIFRSS CPINVLYFPF DWQNCSLKFT ALNYDANEIT MDLMTDTIDG KDYPIEWIII DPEAFTENGE WEIIHKPAKK NIYPDKFPNG TNYQDVTFYL IIRRKPLFYV INFITPCVLI SFLASLAFYL PAESGEKMST AISVLLAQAV FLLLTSQRLP ETALAVPLIG KYLMFIMSLV TGVIVNCGIV LNFHFRTPST HVLSTRVKQI FLEKLPRILH MSRADESEQP DWQNDLKLRR SSSVGYISKA QEYFNIKSRS ELMFEKQSER HGLVPRVTPR IGFGNNNENI AASDQLHDEI KSGIDSTNYI VKQIKEKNAY DEEVGNWNLV GQTIDRLSMF IITPVMVLGT IFIFVMGNFN HPPAKPFEGD PFDYSSDHPR CA |
| Affinity tags | none |
| Expression Host | T. californica (native) |
| Size (excluding additional elements) | 65 kDA |
| Buffer composition | 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 5 % glycerin; 0,1 % Triton |
| Purity | >95%, determined via SDS-PAGE |
| Purified via | Protein-specific affinity matrix using immobilized agonist ligand followed by dialysis |
| Function | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. |
Citations
Lab Results
FAQ
Can I get the datasheet for the AChR protein?
Do you also offer other full-length membrane proteins?
Yes, we do. Just browse through our product protofolio. In case we do not have your desired membrane protein in our store, send a request to our protein services.