Excitatory amino acid transporter 4 (SLC1A6 / EAAT4)

Order number: 70170

€10,800.00*

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Description

We are excited to introduce our full-length, active, and native Excitatory Amino Acid Transporter 4 (EAAT4) protein, encoded by SLC1A6. This essential membrane transporter is involved in maintaining glutamate balance and preventing excitotoxicity, making it a crucial target in neurological research. EAAT4’s role in regulating neurotransmission, particularly in the cerebellum, opens doors for therapeutic applications in neurodegenerative and psychiatric disorders.

Our SLC1A6 protein has been solubilized and stabilized using our NativeMP™ platform, ensuring it retains its native structure and full biological activity, ideal for a variety of research applications.

Applications:

Screening/Validation
Biologics Development
Structure Determination
Small Molecule Drug Development

The protein delivered can be used for these specific applications. When ordering, please indicate which applications you intend to use the protein for in the contact form to ensure the best results.

For more details, explore our solubilization database.

copolymer vs detergents workflow — **Fig. 1:** Detergents create environments that lack native lipids, which can compromise protein function and stability. Copolymers, enable you to see and analyze these aspects within a native environment. They facilitate studying how your target interacts with its surrounding lipids, and allow assays to be conducted at body temperature.

Datasheets

Upon ordering, you will receive the corresponding datasheets and the Certificate of Analysis (CoA).

Feature
Alternative names	SLC1A6, EAAT4
UniProt Number	P48664
Protein class	SLC transporter
Original host	Homo sapiens
Expression system	HEK293
Sequence, One-Letter Code	MSSHGNSLFLRESGQRLGRVGWLQRLQESLQQRALRTRLRLQTMTLEHVLRFLRRNAFILLTVSAVVIGVSLAFALRPYQLTYRQIKYFSFPGELLMRMLQMLVLPLIVSSLVTGMASLDNKATGRMGMRAAVYYMVTTIIAVFIGILMVTIIHPGKGSKEGLHREGRIETIPTADAFMDLIRNMFPPNLVEACFKQFKTQYSTRVVTRTMVRTENGSEPGASMPPPFSVENGTSFLENVTRALGTLQEMLSFEETVPVPGSANGINALGLVVFSVAFGLVIGGMKHKGRVLRDFFDSLNEAIMRLVGIIIWYAPVGILFLIAGKILEMEDMAVLGGQLGMYTLTVIVGLFLHAGIVLPLIYFLVTHRNPFPFIGGMLQALITAMGTSSSSATLPITFRCLEEGLGVDRRITRFVLPVGATVNMDGTALYEALAAIFIAQVNNYELNLGQITTISITATAASVGAAGIPQAGLVTMVIVLTSVGLPTEDITLIIAVDWFLDRLRTMTNVLGDSIGAAVIEHLSQRELELQEAELTLPSLGKPYKSLMAQEKGASRGRGGNESAMGSSGTETSQVAPA
Affinity tags	Rho1D4-tag (C-terminal)
Size (excluding additional elements)	564 amino acids, 62 kDa
Concentration	0.4 - 0.7 mg/ml
Purity	>90%, determined via SDS-PAGE
Purification process	2-step purification
Shipping	Shipment is carried out on dry-ice for temperature stability to ensure the integrity of the product.
Function	Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na⁺ ions and one proton, in parallel with the counter-transport of one K⁺ ion. Mediates Cl^- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na⁺ symport.

Lab Results

SLC1A6 Eluate + DLS — **Figure 2:** Exemplary in-house SDS-Page and DLS data of SLC1A6 NativeMP™ copolymer screens. More in-depth data sets can be accessed upon project initiation.