PolyHunter Agarose (50 % Suspension)

Order number: 19103

€106.00*

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Description

Solubilization of membrane protein using synthetic copolymer nanodiscs has a lot of advantages over traditional membrane protein solubilization methods.
Cube Biotech's PolyHunter Agarose Resins and MagBeads solve one of the few remaining issues with this product. After solubilization, some remaining free copolymer molecules can interfere with the subsequent affinity chromatography. Our PolyHunter products can deplete up to 99% of these interfering copolymer molecules from your solubilizate.

PolyHunter Agarose Resin is compatible with all nanodisc copolymers that CubeBiotech offers. Therefore it is ideally suited to be purchased in conjunction with our Synthetic nanodisc Screening Kit Maxi.

Note: PolyHunter Products are also available as magnetic beads and can be reused up to 5 times. The corresponding regeneration procedure can be found in the matching protocol PDF files.

PolyHunter Beads working principle — **Figure 1:** Schematic depiction of a gravity flow column that depletes a copolymer solubilizate from its remaining free copolymer molecules.

Datasheets

Lab Results

AASTY 6-50 performance compared to other polymers, shown on a gel — **Figure 2:** Average copolymer depletion efficiencies of PolyHunter beads of the four copolymer classes that Cube Biotech offers. Note that the depletion efficiency of DIBMA is lower that the rest. You can solve this problem by performing the PolyHunter protocols twice.

Protein yield increase after PolyHunter treatment of the sample — **Figure 3:** Affinity chromatography (AC) of a 9-transmembrane-domain protein. Each step of the protein purification procedure was performed with and without PolyHunter treatment.
S: Original solubilizates, both of them containing the same amount of protein
**FT:** Flow-through after the binding step of the AC. The Polyhunter treated flow-through contains less protein and therefore more has successfully bound to the affinity beads.
W: Wash fractions (near invisible), in both cases next to no protein was eluted during the washing steps.
E: Elution fractions, PolyHunter treated sample shows a higher protein yield.

AASTY 11-50 depletion efficiency. First use and after regeneration steps — **Figure 4:** Depletion efficiencies of AASTY 11-50 measured at wavelength 260nm. Note that even after 5 regenerations (see protocol) of the PolyHunter beads the depletion efficiency is nearly identical, if not greater than the first "fresh" run.

AASTY 6-50 depletion efficiency. First use and after regeneration steps — **Figure 5:** Depletion efficiencies of AASTY 6-50 measured at wavelength 260nm. Note that even after 5 regenerations (see protocol) of the PolyHunter beads the depletion efficiency is nearly identical, if not greater than the first "fresh" run.

AASTY 6-55 depletion efficiency — **Figure 6:** Depletion efficiency of PolyHunter products of AASTY 6-55. In this depletion, a near 100% efficiency was reached.

FAQ

Can I have the PolyHunter Agarose Resin Datasheet or protocol?

Yes, of course. Simply download them here.

Do PolyHunter beads also deplete AASTY / DIBMA / SMA / Amphipols?

Yes, it does. Small note: The depletion of DIBMA is a little bit less efficient. Therefore, it is recommended to perform the depletion protocol for DIBMA twice.

How often can I reuse PolyHunter beads?

Properly used and regeneration, PolyHunter beads can be reused up to 5 times.