DIBMA 6 Fluorescein

Order number: 18762

€1,418.00*

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Description

The new DIBMA 6 is not only smaller than its predecessors (DIBMA 10 and DIBMA 12) but together with Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and lower polydispersity, meaning that if you need your copolymers to all have a similar size this is the copolymer to choose.

DIBMA 6 Fluorescein

This first-of-its-kind, functionalized version of our classic DIBMA 6 is fluorescently labeled with fluorescein. This means a pH-dependent fluorescent excitation at ~485 nm and emission maximum at ~510 nm.

Suitable applications for fluorescent copolymers:

Detection
Localization (Visualization via fluorescent microscopy)
etc.

If you’re interested in having this functionalization applied to a different copolymer backbone, get in touch with us and we’ll get that project running for you: Contact us

Datasheets

Feature
State	Lyophilized Powder, to be solved with water
Full Name	Diisobutylene Maleic Acid Fluorescein in 50 mM HEPES, pH 7.5
Buffer	HEPES
pH after solving	7.5
pH Stability of Copolymer	5.0-10
Absorbance at 280nm	Yes
Molecular Weight	6 kDA
Ca²⁺ tolerance	30 mM
Mg²⁺ tolerance	40 mM
Solubility	> 10% (H₂O)
Polymerization Type	RAFT
λAbs	~485 nm
λEm	~514 nm
Shipping Temperature	Ambient Temparature
Storage of lyophilized copolymer	-20°C for several years
Storage of dissolved copolymer	2-8°C for several days

Lab Results

Molecular Size Distribution of DIBMA 6 Nanodiscs — Fig. 1: Size distribution of DIBMA 6 copolymers synthesized via RAFT compared to other copolymers synthesized via classical methods. Shown is the reduction in both monomer size and lower polydispersity for RAFT synthesized DIBMA 6.

DIBMA Variants Comparison — Fig. 2: Rho-tagged membrane protein has been solubilized and stabilized from celll membrane in one step at 23°C with an array of DIBMA copolymer variants including the new DIBMA 6 (here without fluorescein functionalization). Protein of interest was purified at 23°C from solubilizate via Rho-Magbeads, eluates were analysed via SDS-Page and Western-blot.

Fig. 3:HPLC Analysis of DIBMA Fluoresceine vs DIBMA 6 with an emission at 510 nm and excitation at 495 nm, 1% Polymer Solution in DMF.

Disclaimer

Patent Pending
Our products are intended for molecular applications. These products are not intended for diagnosis, prevention or treatment of a disease.