SMALP Screening Set (4x3x50 mg)
Order number: 18280
Description
Membrane protein and their composition as as versatile as the whole cell membrane itself. For this reason it is sadly impossible to predict with a 100% accuracy which SMALP product is suited best for the solubilization & subsequent of your membrane protein of interest.
For this reason we offer this SMALP Screening Kit that consists of our four individual SMAs. This way you have a convenient way to screen for the best SMA for your protein before scaling up your assay. In case you would like to extend your polymer screening beyond the reaches of SMA we offer our Synthetic Nanodisc Screening Kit, that covers both SMA and DIBMA.
Datasheets
| Feature | SMALP 140 | SMALP 140-I | SMALP 200 | SMALP 300 |
|---|---|---|---|---|
| Polymer-to-maleic-anhydride ratio [n:m] | 1.4:1 | 1.4:1 | 2:1 | 3:1 |
| Molecular weight | 5 kDa | 5 kDa | 6.5 kDa | 10 kDa |
| Form | Lyophilized powder | Lyophilized powder | Lyophilized powder | Lyophilized powder |
| pH, after solving | 7.5 | 7.5 | 7.5 | 7.5 |
| Divalent cationic tolerance | < 5 mM | < 100 mM | < 5 mM | < 5 mM |
| Color | Amber | Brownish | Yellowish | Colorless |
| Odor | Odorless | Odorless | Odorless | Odorless |
| Other noteworthy feature | Is imidized with DMAPA | Most commonly used SM | ||
| Shipping Temperature | ambient temperature“ | |||
| Storage of lyophilized copolymer | -20°C for several years | |||
| Storage of dissolved copolymer | 2-8°C for several days | |||
Citations
| Stabilized protein | Year | Author |
|---|---|---|
| Bacterial Membrane Proteins | 2022 | Noh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L. |
Lab Results
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set.
Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
Video
Watch our video on the use of synthetic polymers for membrane protein solubilization. It demonstrates the use with SMALP.
FAQ
What SMA is the best one to use?
This is a tricky question, as it depends on the membrane protein which SMA performs best, however, SMA 200 is in general the most recommended. Keep in mind that this is no guarantee for it to be the best but in our experience, it is a good allrounder.
What are the recommended concentrations of SMA?
Mix the supplied solutions at a concentration of 1-5% SMALP to the total solution. These conditions can be used as indications. The optimal SMA concentration must be determined separately for each experiment.
How is SMA stored?
Store in cool and dry places, protected from direct sunlight. Long-term storage is recommended at 4°C
The viscosity of my SMA has decreased, what happene
This can occur by storing SMA at low temperatures (as recommended!). Simply warm the SMA solution up to room temperature to restore its original viscosity.
How do I quantify the amount of protein after SMA treatment?
In contrast to DIBMA, SMA absorbs light at 280 nm similar to proteins. Therefore ultraviolet absorption is no option here. However other protein quantification assays are viable options like:
- Bicinchoninic Acid (BCA)
- Bradford
- Folin-Lowry in some cases
Disclaimer
The use of this styrene maleic acid copolymer (SMA) product for the manufacture of styrene maleic acid copolymer - lipid particles (SMALP), and the use of SMALP, are covered by one or more of the following patents owned by Malvern Cosmeceutics Limited: US 8623414, EP 1890675, GB 2426703, AU 2006253886, JP 5142898, IN 261468, CN ZL200680018957.2 and CA 2,611,144 and by the University of Birmingham: US 8754168.
The purchaser is licensed under those patents to use the SMA for the manufacture of SMALP and to use the SMALP for the purpose of research and development of proteins, including their production (including purification and solubilisation), screening testing, analysis, characterisation (including structural analysis and characterisation), including for the purpose of drug screening and for the purpose of in vitro diagnostics, but not for the purpose of delivery of agents to humans or other animals for therapeutic, diagnostic (including in vivo diagnostics) or prophylactic purposes, which uses are specifically prohibited.
The use of SMALP 502-E within the United States is not covered by our current license and this product can therefore not be made available for US purchasers.
The purchaser is licensed under those patents to use the SMA for the manufacture of SMALP and to use the SMALP for the purpose of research and development of proteins, including their production (including purification and solubilisation), screening testing, analysis, characterisation (including structural analysis and characterisation), including for the purpose of drug screening and for the purpose of in vitro diagnostics, but not for the purpose of delivery of agents to humans or other animals for therapeutic, diagnostic (including in vivo diagnostics) or prophylactic purposes, which uses are specifically prohibited.
The use of SMALP 502-E within the United States is not covered by our current license and this product can therefore not be made available for US purchasers.