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Ultrasolute™ Amphipol 18 Biotin

Order number: 19721
Ultrasolute™ Amphipol 18 Biotin

€907.00*

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Description

This first-of-its-kind, functionalized version of Ultrasolute™ Amphipol 18 is tagged with biotin, enabling tag-free solubilization and stabilization of your target protein. There is no protein-labeling required, and the accesibility to the ecto- and cytoplasmatic domain is preserved.
This functionalized copolymer is particularly suited for downstream applications that require copolymer stabilization without affecting the protein. These include drug screening, high-throughput processes and assay development.

More applications for biotin-tagged copolymers:
  • Immobilization via SPR, ELISA, etc.
  • Sorting (FACS)
  • Quantification
  • Identification
  • Localization (Electron Microscopy)

Find more of our biotinylated copolymers included in the  NativeMP™ Biotin Kit. 

If you’re interested in having this functionalization applied to a different copolymer backbone, get in touch with us and we’ll get that project running for you: Contact us

Datasheets

Feature
State Lyophilized Powder, to be solved with water
Full Name Ultrasolute Amphipol 18 Biotin in 50 mM HEPES, pH 7.5
Buffer HEPES
pH after solving 7.5
pH Stability of Copolymer 5.0-10
Absorbance at 280nm No
Molecular Weight 7.3 kDA
Ca2+ tolerance 25 mM
Mg2+ tolerance 25 mM
Solubility > 10% (H2O)
Polymerization Type Free-radical
Shipping Temperature Ambient Temparature
Storage of lyophilized copolymer -20°C for several years
Storage of dissolved copolymer 2-8°C for several days

Lab Results

NativeMP Biotin Copolymers Rho Blot
Fig. 1: Rho-tagged membrane protein has been solubilized and stabilized from celll membrane in one step with an array of biotinylated copolymers.
DLS Analysis of Streptavidin Binding to P2X4 Stabilized in NativeMP Biotin Copolymers
Fig. 2: DLS analysis reveals the successful binding of strepavidin to the biotinylted copolymer which stabilizes the membrane protein P2X4.
SPR Analysis - P2X4 in Biotinylated Copolymer
Fig. 3 A: P2X4, stabilized in a biotinylated copolymer, was used to investigate its binding interaction with the ligand 5-BDBD using Surface Plasmon Resonance (SPR). The membrane protein was immobilized on a Streptavidin chip. After establishing a baseline, the signal increased during the association phase, indicating successful binding of the biotin on the copolymer binding to the streptavidin on the chip. In the dissociation phase, the unbound protein was removed, leaving no free biotinylted copolymer-protein nanodisc in the solution, resulting in a decrease in signal.
SPR Analysis - P2X4 in Biotinylated Copolymer
Fig. 3 B: Following the immobilization of P2X4 on the chip, the analyte 5-BDBD at two concentrations (1 µM and 10 µM) was used to determine the dissociation constant (Kd). The increase in signal during the association phase indicates sucessful binding between the analyte and P2X4, while the decrease in signal in the dissociation phase reflects complex retention. The resulting curves where fitted, and Kd value of 1.6 µM was estimated.

Disclaimer

Our products are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.