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NativeMP™ Biotin Kit (6x1x50mg)

Order number: 19791
NativeMP™ Biotin Kit (6x1x50mg)
NativeMP™ Biotin Kit (6x1x50mg)
NativeMP™ Biotin Kit (6x1x50mg)
NativeMP™ Biotin Kit (6x1x50mg)

€900.00*

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Description

The NativeMP™ Biotin Kit leverages a curated copolymer selection from the NativeMP™ Technology, which preserves membrane proteins in their native lipid environment without detergents. These copolymers are additionally labeled with biotin and are well suited for immobilization and downstream applications such as SPR, ELISA, FACS, and various high-throughput assays, making them ideal for drug discovery and assay development.  
The copolymers are already biotinylated so that you can work with unmodified membrane proteins and avoid the extra steps necessary to label your proteins. This allows you to focus on expressing unmodified membrane proteins that can be stabilized with the biotinylated copolymers and used directly in any number of immobilization or downstream applications. 
The NativeMP™ Biotin Kit offers a targeted solution for precise stabilization and solubilization without additional protein labeling.

Screening is key

Each target is unique, which is why screening is just as important for biotin copolymers as it is for detergents. Our NativeMP™ Biotin Kit offers a selection of 6 biotinylated copolymers, ensuring that you’ll find the right copolymer to meet the specific needs of your target and downstream applications.

If you’re interested in having this functionalization applied to a different copolymer, get in touch with us, and we’ll get that project running for you: Contact us

Datasheets

Table 1: Shared Features

All the biotin copolymers featured in this kit share the following features
Feature  
Use Solubilization & stabilization of membrane proteins
Buffer HEPES
pH (upon solving in dH2O) 7.5
State upon delivery Lyophilized powder
Recommended concentration for use 1% - 5%
Shipping Temperature Room temperature
Long-term Storage (lyophilized copolymer) -20°C for several years
Short-term Storage (dissolved copolymer) 2°C - 8°C for several days


Table 2: Included Biotin Copolymers

Feature Ultrasolute Amphipol 18 Biotin Cubipol Biotin Sulfo-Cubipol Biotin DIBMA 6 Biotin AASTY 6-50 Biotin AASTY 11-50 Biotin
Molecular weight 7,3 kDa 7,4 kDa 14 kDa 6 kDa 6 kDa 11 kDa
Polymerization Type Free-Radical Free-Radical Free-Radical RAFT RAFT RAFT
Divalent Cationic Tolerance < 25 mM < 40 mM < 40 mM < 40 mM < 6 mM < 6 mM
Absorption at 280nm No No No No Yes Yes
Solubility in dH2O <10% < 10% <10% <10% <10% <10%


Lab Results

NativeMP Biotin Copolymers Rho Blot
Fig. 1: Rho-tagged membrane protein has been solubilized and stabilized from celll membrane in one step with an array of biotinylated copolymers.
DLS Analysis of Streptavidin Binding to P2X4 Stabilized in NativeMP Biotin Copolymers
Fig. 2: DLS analysis reveals the successful binding of strepavidin to the biotinylted copolymer which stabilizes the membrane protein P2X4.
SPR Analysis - P2X4 in Biotinylated Copolymer
Fig. 3 A: P2X4, stabilized in a biotinylated copolymer, was used to investigate its binding interaction with the ligand 5-BDBD using Surface Plasmon Resonance (SPR). The membrane protein was immobilized on a Streptavidin chip. After establishing a baseline, the signal increased during the association phase, indicating successful binding of the biotin on the copolymer binding to the streptavidin on the chip. In the dissociation phase, the unbound protein was removed, leaving no free biotinylted copolymer-protein nanodisc in the solution, resulting in a decrease in signal.
SPR Analysis - P2X4 in Biotinylated Copolymer
Fig. 3 B: Following the immobilization of P2X4 on the chip, the analyte 5-BDBD at two concentrations (1 µM and 10 µM) was used to determine the dissociation constant (Kd). The increase in signal during the association phase indicates sucessful binding between the analyte and P2X4, while the decrease in signal in the dissociation phase reflects complex retention. The resulting curves where fitted, and Kd value of 1.6 µM was estimated.

Disclaimer

Our products are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.