Green fluorescent protein (eGFP)


Purified Enhanced Green Fluorescent Protein (eGFP)

Green Fluorescent Protein is a natural, autofluorescent protein from the jellyfish Aequorea victoria that is frequently used as a control protein in method development and calibration. Enhanced GFP (eGFP) is a mutated GFP with enhanced fluorescence signal and a slightly shifted excitation spectrum. Cube Biotech offers three variants of eGFP with His, GST, Rho1D4, and DYKDDDDK-(also known as FLAG-) tags. Please contact us for bulk inquiries, academic projects or collaboration ideas.
Expression system E. coli
Purification affinity chromatography
Provided as lyophilized from 10 mM Tris-HCl, pH 4.0
Excitation maximum 488 nm
Emission maximum 509 nm
GFP on SDS Page
Fig. 1: Size and purity of three eGFP variants assessed by SDS-PAGE.
ProteinProtein sizeProtein sequence
    Red: His-tag, Grey Underlined: Spacer, Blue: GST-tag, , Light Green: Rho1D4-tag, Dark Green: DYKDDDDK-tag, Purple: Thrombin site, Black: eGFP, Underlined: HRV 3C (PreScission) site
His-eGFP 26 kDa MKHHHHHHHMRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQL ADHYQQNTPI GDGPVLLPDN HYLSTQSALS KDPNEKRDHM VLLEFVTAAG ITHGMDELYKStop
GST-eGFP-Rho1D4 55.4 kDa MSPILGYWKIK GLVQPTRLLL EYLEEKYEEH LYERDEGDKW RNKKFELGLE FPNLPYYIDG DVKLTQSMAI IRYIADKHNM LGGCPKERAE ISMLEGAVLD IRYGVSRIAY SKDFETLKVD FLSKLPEMLK MFEDRLCHKT YLNGDHVTHP DFMLYDALDV VLYMDPMCLD AFPKLVCFKK RIEAIPQIDK YLKSSKYIAW PLQGWQATFG GGDHPPKSD LVPRGS LEVLFQGP MRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQL ADHYQQNTPI GDGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI THGMDELYKGSSG TETSQVAPAStop
GST-eGFP-DYKDDDDK 55.5 kDa MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGS LEVLFQGP MRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQ LADHYQQNT PIGDGPVLLP DNHYLSTQSA LSKDPNEKRD HMVLLEFVTA AGITHGMDEL YKGSSG DYKDDDDKStop
Top seller
GST-eGFP fusion protein, DYKDDDDK-tag GST-eGFP fusion protein, DYKDDDDK-tag
Article number: 29921
eGFP protein, His-tag eGFP protein, His-tag
Article number: 29901
GST-eGFP fusion protein, Rho1D4-tag GST-eGFP fusion protein, Rho1D4-tag
Article number: 29911
Green fluorescent protein (eGFP)
Close filters
No results were found for the filter!
GST-eGFP fusion protein, DYKDDDDK-tag GST-eGFP fusion protein, DYKDDDDK-tag
GST-eGFP fusion protein with the commonly DYKDDDDK / FLAG-tag. Pure and ready to be used for your research.
Article number: 29921
Sales price: From €226.00 *
Details
eGFP protein, His-tag eGFP protein, His-tag
The enhanced green fluorescent protein (eGPF) is an improvement of the already very versatile GFP. 100% pure & His-tagged.
Article number: 29901
Sales price: From €200.00 *
Details
GST-eGFP fusion protein, Rho1D4-tag GST-eGFP fusion protein, Rho1D4-tag
GST-eGFP protein with Rho1D4-tag. | For positive controls of purification assays. | Pure and ready for your research.
Article number: 29911
Sales price: From €226.00 *
Details
Viewed
This website uses cookies to ensure you get the best experience on our website. More information
Accept