Purified Enhanced Green Fluorescent Protein (eGFP)
Green Fluorescent Protein is a natural, autofluorescent protein from the jellyfish Aequorea victoria that is frequently used as a control protein in method development and calibration. Enhanced GFP (eGFP) is a mutated GFP with enhanced fluorescence signal and a slightly shifted excitation spectrum. Cube Biotech offers three variants of eGFP with His, GST, Rho1D4, and DYKDDDDK (also known as FLAG) tags.
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| Expression system |
E. coli |
| Purification |
affinity chromatography |
| Provided as |
lyophilized from 10 mM Tris-HCl, pH 4.0 |
| Excitation maximum |
488 nm |
| Emission maximum |
509 nm |
Fig. 1: Size and purity of three eGFP variants assessed by SDS-PAGE.
| Protein | Protein size | Protein sequence |
|---|
| |
|
Red: His-tag, Grey Underlined: Spacer, Blue: GST tag, , Light Green: Rho1D4 tag, Dark Green: DYKDDDDK tag, Purple: Thrombin site, Black: eGFP, Underlined: HRV 3C (PreScission) site |
| His-eGFP |
26 kDa |
MKHHHHHHHMRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQL ADHYQQNTPI GDGPVLLPDN HYLSTQSALS KDPNEKRDHM VLLEFVTAAG ITHGMDELYKStop |
| GST-eGFP-Rho1D4 |
55.4 kDa |
MSPILGYWKIK GLVQPTRLLL EYLEEKYEEH LYERDEGDKW RNKKFELGLE FPNLPYYIDG DVKLTQSMAI IRYIADKHNM LGGCPKERAE ISMLEGAVLD IRYGVSRIAY SKDFETLKVD FLSKLPEMLK MFEDRLCHKT YLNGDHVTHP DFMLYDALDV VLYMDPMCLD AFPKLVCFKK RIEAIPQIDK YLKSSKYIAW PLQGWQATFG GGDHPPKSD LVPRGS LEVLFQGP MRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQL ADHYQQNTPI GDGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI THGMDELYKGSSG TETSQVAPAStop |
| GST-eGFP-DYKDDDDK |
55.5 kDa |
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGS LEVLFQGP MRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQ LADHYQQNT PIGDGPVLLP DNHYLSTQSA LSKDPNEKRD HMVLLEFVTA AGITHGMDEL YKGSSG DYKDDDDKStop |
