Green fluorescent protein (eGFP)

Purified Enhanced Green Fluorescent Protein (eGFP)

Green Fluorescent Protein is a natural, autofluorescent protein from the jellyfish Aequorea victoria that is frequently used as a control protein in method development and calibration. Enhanced GFP (eGFP) is a mutated GFP with enhanced fluorescence signal and a slightly shifted excitation spectrum. Cube Biotech offers three variants of eGFP with His, GST, Rho1D4, and DYKDDDDK (also known as FLAG) tags. Please contact us for bulk inquiries, academic projects or collaboration ideas.

Expression system	E. coli
Purification	affinity chromatography
Provided as	lyophilized from 10 mM Tris-HCl, pH 4.0
Excitation maximum	488 nm
Emission maximum	509 nm

Fig. 1: Size and purity of three eGFP variants assessed by SDS-PAGE.

Protein

Protein size

Protein sequence

Red: His-tag, Grey Underlined: Spacer, Blue: GST tag, , Light Green: Rho1D4 tag, Dark Green: DYKDDDDK tag, Purple: Thrombin site, Black: eGFP, Underlined: HRV 3C (PreScission) site

His-eGFP

26 kDa

MKHHHHHHHMRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQL ADHYQQNTPI GDGPVLLPDN HYLSTQSALS KDPNEKRDHM VLLEFVTAAG ITHGMDELYKStop

GST-eGFP-Rho1D4

55.4 kDa

MSPILGYWKIK GLVQPTRLLL EYLEEKYEEH LYERDEGDKW RNKKFELGLE FPNLPYYIDG DVKLTQSMAI IRYIADKHNM LGGCPKERAE ISMLEGAVLD IRYGVSRIAY SKDFETLKVD FLSKLPEMLK MFEDRLCHKT YLNGDHVTHP DFMLYDALDV VLYMDPMCLD AFPKLVCFKK RIEAIPQIDK YLKSSKYIAW PLQGWQATFG GGDHPPKSD LVPRGS LEVLFQGP MRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQL ADHYQQNTPI GDGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI THGMDELYKGSSG TETSQVAPAStop

GST-eGFP-DYKDDDDK

55.5 kDa

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGS LEVLFQGP MRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQ LADHYQQNT PIGDGPVLLP DNHYLSTQSA LSKDPNEKRD HMVLLEFVTA AGITHGMDEL YKGSSG DYKDDDDKStop

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Green fluorescent protein (eGFP)

Purified Enhanced Green Fluorescent Protein (eGFP)

Expression system	E. coli
Purification	affinity chromatography
Provided as	lyophilized from 10 mM Tris-HCl, pH 4.0
Excitation maximum	488 nm
Emission maximum	509 nm

Fig. 1: Size and purity of three eGFP variants assessed by SDS-PAGE.

Protein

Protein size

Protein sequence

Red: His-tag, Grey Underlined: Spacer, Blue: GST tag, , Light Green: Rho1D4 tag, Dark Green: DYKDDDDK tag, Purple: Thrombin site, Black: eGFP, Underlined: HRV 3C (PreScission) site

His-eGFP

26 kDa

GST-eGFP-Rho1D4

55.4 kDa

GST-eGFP-DYKDDDDK

55.5 kDa

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGS LEVLFQGP MRSGKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGDAT YGKLTLKFIC TTGKLPVPWP TLVTTLTYGV QCFSRYPDHM KQHDFFKSAM PEGYVQERTI FFKDDGNYKT RAEVKFEGDT LVNRIELKGI DFKEDGNILG HKLEYNYNGH NVYITADKQK NGIKANFKIR HNIEDGSVQ LADHYQQNT PIGDGPVLLP DNHYLSTQSA LSKDPNEKRD HMVLLEFVTA AGITHGMDEL YKGSSG DYKDDDDKStop

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