PureCube NTA agarose resin columns were developed for IMAC based purification and enrichment methods. Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. By providing our coupling protocols, feel free to create any NTA purification resin you desire. PureCube NTA resin cartridges are pre-filled FPLC columns that contain our PureCube NTA agarose. The cartridges were designed for maximum compability with most FPLC hardware. Please check the given features table to confirm their compability with your hardware. For the rare case incompability between our cartridges and your FPLC machinery have a look at our PureCube NTA COMPACT cartridges. They were designed for this kind of situation. Furthermore PureCube NTA can also be purchased magnetic beads / MagBeads.
Features - Regarding the resin.
Usage
For coupling of metal ions for corresponding purification and / or enrichment purposes
Specifity
Varies
Binding capacity
Depends on the loaded ion
Purity
Depends on the loaded ion
Bead Ligand
NTA
Bead size
40 μm
Matrix
7.5% cross-linked agarose
Recommended FPLC flow rates
0.5-2 mL/min
Maximal FPLC flow rate
6 mL/min
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
