PureCube Compact Cartridge NTA
Order number: 31908
Description
The PureCube NTA (nitrilotriacetic acid) Compact Cartridge was developed for IMAC-based protein purification and protein enrichment methods. The beads in the pre-packed resin have an average diameter of 40 µm. This small sized provides great mechanical stability.
The Purecube NTA agarose resin is packed into our PureCube Compact Cartridge. The Compact Cartridge is designed for compability with most commonly used FPLC systems like AEKTAs.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.
However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
The Purecube NTA agarose resin is packed into our PureCube Compact Cartridge. The Compact Cartridge is designed for compability with most commonly used FPLC systems like AEKTAs.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.
However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
- His-tag purification: Ni-NTA, Co-NTA, Zn-NTA, and Cu-NTA
- Phosphopeptide Enrichment: Al-NTA, Fe-NTA, Ti-NTA, and Zr-NTA. (Ti and Zr only as MagBeads)
Datasheets
| Feature NTA Resin | |
|---|---|
| Usage | For coupling of metal ions for corresponding purification and / or enrichment purposes |
| Specificity | Varies |
| Binding capacity | Depends on the loaded ion |
| Purity | Depends on the loaded ion |
| Bead Ligand | NTA |
| Bead size | 40 μm |
| Matrix | 7.5% cross-linked agarose |
| Filling quantity | Delivered as a 50 % suspension |
| Recommended FPLC flow rates | 0.5-2 ml/min |
| Maximal FPLC flow rate | 6 ml/min |
| pH stability | 2-14 |
| Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
| Feature - Column | ||
|---|---|---|
| PureCube Compact Cartridge 1 mL | PureCube Compact Cartridge 5 mL | |
| Bed Volume | 1 mL | 5 mL |
| Max. Flow Rate | 6 mL/min | 6 mL/min |
| Dimensions: diameter X length (mm) | 5 X 35 | 17 X 35 |
| Body material | Polypropylene | Polypropylene |
| Inlet | 10-32 UNF female thread | 10-32 UNF female thread |
| Outlet | 10-32 UNF female thread | 10-32 UNF female thread |
FAQ
Can I get the datasheet for the PureCube NTA Compact Cartridge?
Why is there a special protocol for the loading of nickel ions?
As Ni-NTA is the most commonly used NTA product, we have optimized this specific protocol.
Can I order a specific NTA product from you, even if it is not in your shop?
Yes, we offer a custom bead service for that.