PureCube Compact Cartridge Glutathione
Order number: 32302
Description
The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. This is why GST-tag purifications exploit this specificity to gain high levels of purity while maintaining a high protein yield. The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTA TM.
The PureCube Glutathione Compact cartridge is a FPLC column pre-packed with PureCube Glutathione Agarose. Alternatively, Glutathione MagBeads are also available, if you prefer this affinity chromatography approach.
Additional support products for GST-tag purification assays are L-Glutathione (reduced), an essential compound of the elution buffer, and our GST-tag antibody for later western blot detection of your protein.
The PureCube Glutathione Compact cartridge is a FPLC column pre-packed with PureCube Glutathione Agarose. Alternatively, Glutathione MagBeads are also available, if you prefer this affinity chromatography approach.
Additional support products for GST-tag purification assays are L-Glutathione (reduced), an essential compound of the elution buffer, and our GST-tag antibody for later western blot detection of your protein.
Datasheets
Feature - Glutathione Resin | |
---|---|
Usage | Specific binding and purification of GST-tagged proteins |
Specificity | Affinity to GST(Gluthathione-S-Transferase)-tagged proteins |
Binding capacity | >10 mg/ml |
Bead Ligand | L-Glutathione (reduced) |
Bead size | 40 μm |
Matrix | 7.5% cross-linked agarose |
Filling quantity | Delivered as a 50 % suspension |
pH Stability | 3-12 |
Required equipment & materials |
|
Feature - Column | ||
---|---|---|
PureCube Compact Cartridge 1 mL | PureCube Compact Cartridge 5 mL | |
Bed Volume | 1 mL | 5 mL |
Max. Flow Rate | 6 mL/min | 6 mL/min |
Dimensions: diameter X length (mm) | 5 X 35 | 17 X 35 |
Body material | Polypropylene | Polypropylene |
Inlet | 10-32 UNF female thread | 10-32 UNF female thread |
Outlet | 10-32 UNF female thread | 10-32 UNF female thread |
Lab Results
Lab funds are sadly often the limiting factor when it comes to research. So it is of utmost importance that one gets the most value from every purchase one does. The reusability of products and materials is a key word here!
Our GST-tag purification products are able to reproduce all purification assays from lot to lot. We achieved minimal fluctuations between multiple uses of our beads. Multiple consecutive GST-tag purification with the same beads is ensured to be of the same high quality.
Our GST-tag purification products are able to reproduce all purification assays from lot to lot. We achieved minimal fluctuations between multiple uses of our beads. Multiple consecutive GST-tag purification with the same beads is ensured to be of the same high quality.
FAQ
Can I get the datasheet for the PureCube Glutathione Compact Cartridge?
Why are there beads of different sizes for the same purpose?
It is a balance to strike here between protein yield (smaller beads) and other factors like higher FPLC flow rates or handling of very viscous cell media (bigger beads).