PureCube HiCap StrepTactin MagBeads

Order number: 34290

€5,592.00*

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Volume

Quantity

Description

Our PureCube Strep-Tactin® MagBeads is a suspension of 6% cross-linked magnetic agarose beads that is coupled to the Strep-Tactin® protein. They enable protein yields of up to 7 mg protein per ml MagBeads.

They were developed for protein purification assays using the Strep-Tactin® using magnetic separation. PureCube Strep-Tactin® products can also be obtained as agarose resin. Furthermore, PureCube StrepTactin® MagBeads XL are also available for very vicious cell media.

For your convenience Cube Biotech also offers its own MagBead separator for use with our magnetic beads.

Datasheets

Feature
Usage	Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins
Specificity	Affinity to Strep-tagged (WSHPQFEK) proteins
Binding capacity	>7 mg/ml
Bead Ligand	StrepTactin®
Bead size	30 μm
Matrix	6% cross-linked agarose
Filling quantity	Delivered as a 5 % suspension
Chemical stability	2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH₄)SO₄, 1M CaCl₂, 25% Glycerol
Short-term storage	In equilibration buffer (see protocol)
Long-term storage	In 20% ethanol at 4°C

Citations

Purified Protein	Year	Author
NPTX1	2020	Sticco M.J., Palomino P.A.P., Lukacsovich D., Thompson B.L., Földy C., Ressl S., Martinelli D.C.
Sarm1	2021	Li Y., Pazyra-Murphy M.F., Avizonis D., de Sá Tavares Russo M., Tang S., Chen C-Y., Hsueh Y-P., Bergholz S.J., Jiang T., Zhao J. J:, Zhu J., Woo Ko K., Milbrandt J., DiAntonio A., Segal R.A.
NMNAT	2022	Dingwall C.B ., Strickland A., Yum S.W., Yim A.K., Zhu J., Wang P.L., Yamada Y., Schmidt R.E, Sasaki Y., Bloom A.J., DiAntonio A., Milbrandt J.
TIR domains and TIR domain-encoding proteins	2022	Weagley J.S., Zaydman M., Venkatesh S., Sasaki Y., Damaraju N., Yenkin A., Buchser W., Rodionov D.M. Osterman A., Ahmed T., Barratt M.J., DiAntonio A., Milbrandt J., Gordon J.I.

FAQ

Can I get the datasheet for the PureCube HiCap StrepTactin® MagBeads?

Yes. Just click here to download it as a PDF File.

Is StrepTactin® the same as Streptavidin?

No, they are not. StrepTactin® is a bioengineered protein, originally based on Streptavidin. However, StrepTactin cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.

Can I regenerate & reuse your StrepTactin® beads?

Yes, you can! Simply follow our protocol.

Disclaimer

StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".