PureHT™ Strep-Tactin®XT Plate (KingFisher™ 96 Deep-Well)

Order number: 90130

€693.00*

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Description

PureHT™ Strep-Tactin®XT Plates feature IBA Lifesciences’ high-affinity Strep-Tactin®XT resin — now integrated into Cube’s patented dried-bead plate technology. Optimized for the purification of Twin-Strep-tagged proteins, these 96-deep-well plates deliver highly reproducible results across wells and conditions, enabling efficient screening and scale-up. With a total of 480 µl of pure magnetic beads per plate (at 5 µl per well), you have everything you need for a successful purification.

Compatibility and Customization
Compatible with the KingFisher Flex system, these plates provide a reliable foundation for high-throughput protein purification.

Beyond ready-made Strep-Tactin®XT plates, Cube Biotech offers customized solutions: select your preferred resin, plate type, and bead volume per well. Fill out our PureHT™ Custom Plate Request Form to configure the ideal plate for your workflow.

KingFisher™ 96 Deep-Well Plates
KingFisher-format plates are designed for seamless integration with Thermo Fisher’s KingFisher Flex system, ensuring optimal compatibility and reproducibility in automated magnetic bead-based purification.

For more prices and information, contact our Sales Representatives and they will help you through the entire process via contact@cube-biotech.com.

Datasheets

Feature	PureHT™ Strep-Tactin®XT Plate
Usage	Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Application	High-throughput protein purification in 96 well format
Specificity	Twin-Strep-tag® and Strep-tag®II
Binding capacity	Up to 212 µg protein per well. Binding capacity was determined with a 50 kDa Twin-Strep-tag® fusion protein. Please note that the binding capacity is protein dependent.
Bead Ligand	Strep-Tactin®XT
Bead size	20-40 µm (30 µm average)
Matrix	6% agarose, crosslinked
Bead amount	5 µl dried magnetic beads per well
Stability	6 months after shipping
Storage Conditions	2-8°C
Shipping Conditions	Room temperature

Lab Results

Heatmap of relative yield after purification with PureHT Strep-TactinXT Plates — Fig. 1: Heatmap of relative yields after the purification of a Twin -Strep-tagged protein with PureHT^TM Strep-Tactin®XT Plates. The high reproducibility is showcased by a low well-to-well variability (CV ≤5%).

Multiple Screening Conditions on PureHT Plate — Fig. 2: 96-well plate setup for the protein purification screening of Twin-Strep-tagged mitogen-activated protein kinase 14 (MAPK14/p38-α) using a PureHT^TM Strep-Tactin®XT plate. This way, the influence of different buffer conditions (salt concentration and pH value) as well as varying pellet dilution/lysate viscosity could be screened in parallel.

Disclaimer

Strep-Tactin® and Strep-tag® are trademarks of IBA GmbH. Strep-Tactin® Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".