Strep antibody (0.1 mg)
Order number: 40070
Description
This is an antibody specifically for targeting a Strep-(WSHPQFEK)-tag on a protein. It can be used in westernblots and ELISAs. It's origin is mouse and should be stored at -20°C for the long-term.
Datasheets
| Feature | |
|---|---|
| Antibody Name | Strep antibody |
| Cell Line / Origin | Mouse Hybridoma Produced using Strep-tag II peptide conjugated to keyhole limpet hemocyanin as immunogen |
| Subclass | IgG | Suitable for | Western blot, ELISA, FACS, Immune fluorescence |
| Form | 100 µg lyophilized from PBS solution (2.7 mM KCl, 1.5 mM KH2HPO4, 6.5 mM Na2HPO4, 137 mM NaCl, pH 7.3) |
| Reconstitution of lyophilized antibody | Reconstitute antibody in PBS buffer at concentrations between 0.2 and 1.0 mg/ml (i.e. dissolve with 0.5 to 0.1 PBS) |
| Recommended dilution for Western Blot, chromogenic detection | 0.2-0.5 µg/ml antibody diluted in PBS containing 2% BSA. Use rabbit anti-mouse conjugated to peroxidase as secondary antibody. |
| Recommended dilution for Western Blot, chemiluminescence detection | 20 ng/ml antibody diluted in PBS |
| Recommended dilution for FACS | 10 µg/ml diluted in PBS, using e.g. goat anti mouse IgG AB conjugated to Cy2 (GE Healthcare, 1:400 dilution) |
| Recommended dilution for IF | 10 µg/ml – 5 µg/ml antibody diluted in PBS (use secondary anti-mouse antibody) |
| Shipment Temperature | Room temperature |
| Storage | Store as lyophilized powder for up to 12 months at 2-8°C. Alternatively, aliquot contents and freeze at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles. |
Disclaimer
Strep-Tactin® and Strep-tag® are trademarks of IBA GmbH. PureCube Strep-Tactin® Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".