PureCube HiCap StrepTactin Agarose

PureCube HiCap StrepTactin Agarose
€73.00 *

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  • 34101
The term Strep®-tag refers to an eight amino acid long (WSHPQFEK) protein affinity tag that... more
Product information "PureCube HiCap StrepTactin Agarose"
Type: Agarose
Bead size: 100 µm (Agarose)
Affinity : Strep-tag
Purpose: Protein Purification
The term Strep®-tag refers to an eight amino acid long (WSHPQFEK) protein affinity tag that can be used for affinity chromatography based protein purification. It is based in the increadible specificity of the protein StrepTactin ® that interacts with the specific amino acid squence WSHPQFEK. Exploiting this incredible specificity maximum protein puritiy levels through affinity chromatography are possible.
Our PureCube StrepTactin agarose resin beads consists of 7.5% cross-linked agarose that are coupled to the StrepTactin protein. They enable protein yields of up to 5 mg protein per ml resin. They were developed for FPLC and batch spin based protein purification. PureCube StrepTactin® agarose can also be purchased pre-packed in cartridges and columns. Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
 

Features

 
Usage Highly specific affinity purification of Strep®(WSHPQFEK)-tagged proteins
Specifity Affinity to Strep®-tagged proteins (WSHPQFEK)
Binding capacity 5 mg / ml resin
Bead Ligand StrepTactin ®
Bead size 100 μm
Stability 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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