PureCube HiCap Streptactin Agarose XL
Order number: 57303
Description
Our PureCube HiCap Strep-Tactin® agarose XL resin beads consist of 6% cross-linked agarose that is coupled to the Strep-Tactin® protein. They enable protein yields of up to 5 mg protein per ml resin.
They were developed for FPLC and batch spin-based protein purification. Furthermore, PureCube StrepTactin® agarose resin is also available with smaller agarose beads for higher protein yield and more mechanical stability. Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
They were developed for FPLC and batch spin-based protein purification. Furthermore, PureCube StrepTactin® agarose resin is also available with smaller agarose beads for higher protein yield and more mechanical stability. Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
Datasheets
| Feature | |
|---|---|
| Usage | Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins |
| Specificity | Affinity to Strep-tagged (WSHPQFEK) proteins |
| Binding capacity | >5 mg/ml |
| Bead Ligand | StrepTactin® |
| Bead size | 400 μm |
| Matrix | 6% cross-linked agarose |
| Filling quantity | Delivered as a 50 % suspension |
| Chemical stability | 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol |
| Short-term storage | In neutral buffer at 4°C |
| Long-term storage | In neutral buffer with sodium azide at 4°C |
| Required equipment |
|
FAQ
Can I get the datasheet for the PureCube HiCap StrepTactin® Agarose XL?
Is StrepTactin® the same as Streptavidin?
No, they are not. StrepTactin® is a bioengineered protein, originally based on Streptavidin. However, StrepTactin cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.
Can I regenerate & reuse your StrepTactin® beads?
Disclaimer
StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".