PureCube HiCap Streptactin Agarose XL

PureCube HiCap Streptactin Agarose XL
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The Strep®-tag (WSHPQFEK) is a nine amino acid long protein affinity tag. It is commonly... more
Product information "PureCube HiCap Streptactin Agarose XL"
Type: Agarose
Bead size: 400 µm (XL Agarose)
Affinity : Strep-tag
Purpose: Protein Purification
The Strep®-tag (WSHPQFEK) is a nine amino acid long protein affinity tag. It is commonly used for affinity chromatography based protein purification. The main principle behind this tag's incredible specificity is the protein StrepTactin ® that interacts with the specific amino acid squence WSHPQFEK. By taking advantage of this incredible specificity maximum protein puritiy through affinity chromatography are in reach.
Our PureCube StrepTactin Agarose resin XL beads consist of 7.5% cross-linked agarose that are coupled to the StrepTactin®-tag. They enable protein yields of up to 5 mg protein per ml resin. They were developed for FPLC and batch spin based protein purification. The special size of the XL beads (~400 μm) greatly improves the flow rates during FPLC assays. PureCube StrepTactin® Agarose can also be purchased regular sized (~100 µm). Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
 

Features

 
Usage Highly specific affinity purification of Strep® (WSHPQFEK) tagged proteins
Specifity Affinity to Strep® tagged proteins (WSHPQFEK)
Binding capacity 5 mg / ml Resin
Bead Ligand StrepTactin ®
Bead size 400 μm
Stability 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol
Filling quantity Delivered as a 50 % suspension
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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