
- Order number: 57303
The Strep®-tag (WSHPQFEK) is a nine amino acid long protein affinity tag. It is commonly... more
Product information "PureCube HiCap Streptactin Agarose XL"
The Strep®-tag (WSHPQFEK) is a nine amino acid long protein affinity tag. It is commonly used for affinity chromatography based protein purification. The main principle behind this tag's incredible specificity is the protein StrepTactin ® that interacts with the specific amino acid squence WSHPQFEK. By taking advantage of this incredible specificity maximum protein puritiy through affinity chromatography are in reach.
Our PureCube StrepTactin Agarose resin XL beads consist of 7.5% cross-linked agarose that are coupled to the StrepTactin®-tag. They enable protein yields of up to 5 mg protein per ml resin. They were developed for FPLC and batch spin based protein purification. The special size of the XL beads (~400 μm) greatly improves the flow rates during FPLC assays. PureCube StrepTactin® Agarose can also be purchased regular sized (~100 µm). Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
Our PureCube StrepTactin Agarose resin XL beads consist of 7.5% cross-linked agarose that are coupled to the StrepTactin®-tag. They enable protein yields of up to 5 mg protein per ml resin. They were developed for FPLC and batch spin based protein purification. The special size of the XL beads (~400 μm) greatly improves the flow rates during FPLC assays. PureCube StrepTactin® Agarose can also be purchased regular sized (~100 µm). Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
Features
Usage | Highly specific affinity purification of Strep® (WSHPQFEK) tagged proteins |
Specifity | Affinity to Strep® tagged proteins (WSHPQFEK) |
Binding capacity | 5 mg / ml Resin |
Bead Ligand | StrepTactin ® |
Bead size | 400 μm |
Stability | 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol |
Filling quantity | Delivered as a 50 % suspension |
Required equipment |
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