PureCube Fe-NTA MagBeads
Order number: 31501-Fe
Description
PureCube Fe-NTA MagBeads were developed to enrich phosphorylated proteins. They can also be used to purify his-tagged proteins as a secondary function. The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Fe-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding a high degree of reproducibility. The phosphopeptide enrichment can be automated as it has already been proven in this paper.
As an alternative, we also offer PureCube Fe-NTA Agarose resin and said resin pre-packed in cartridges/columns.
As an alternative, we also offer PureCube Fe-NTA Agarose resin and said resin pre-packed in cartridges/columns.
User feedback by Thomas Perkins JR., Proteomics & 3D Genome Organization, University of Washington
''I specifically enjoy the Fe-NTA MagBeads for a variety of reasons:
''I specifically enjoy the Fe-NTA MagBeads for a variety of reasons:
- Enhanced sensitivity, specificity, and deeper insights into phosphorylation dynamics through improved identification and quantification of phosphorylated peptides
- Easily automated (Great example from the Villén lab at UW led by Dr. Mario Leutert! - https://pubmed.ncbi.nlm.nih.gov/31885202/)
- Reusable! Strip these beads and reuse them; ensure to implement QC for testing the enrichment efficiency after stripping
- Originally optimized on yeasts, but we have found that they work as well for human cell lines and tissues''
Feature | |
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Usage |
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Specificity |
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Bead ligand | Fe-NTA |
Bead size | 30 µm |
Filling quantity | Delivered as a 25 % suspension |
Chelator stability | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
pH stability | 2-14 |
Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
Citations
Lab Results
Automatic Phosphoproteomics for high-throughput projects
Fe-NTA magnetic beads by Cube Biotech have been proven to increase the speed of a high throughput experiment drastically. Leutert et al. (2019) presented the application of PureCube Fe-NTA MagBeads in a procedure that they named R2-P2, which is short for Rapid-Robotic PhosphoProteomics. They used a KingFisherTM Flex for their robotic runs to fully automize the phosphopeptide enrichment process.
Fe-NTA magnetic beads by Cube Biotech have been proven to increase the speed of a high throughput experiment drastically. Leutert et al. (2019) presented the application of PureCube Fe-NTA MagBeads in a procedure that they named R2-P2, which is short for Rapid-Robotic PhosphoProteomics. They used a KingFisherTM Flex for their robotic runs to fully automize the phosphopeptide enrichment process.

Source: Leutert et al. (2019)
Superiority over other phosphopeptide enrichment methods
Leutert et al. compared three different types of IMAC beads (including our PureCube Fe-NTA) and TiO2 microspheres. As it can be seen in figure 2 our PureCube Fe-NTA magnetic beads presented themselves to be the best option for phosphopeptide enrichment. With our Fe-NTA MagBeads the most unique phosphopeptides (Fig. 2 A and C) were enriched with the highest efficiency (Figure 2 B).
Leutert et al. compared three different types of IMAC beads (including our PureCube Fe-NTA) and TiO2 microspheres. As it can be seen in figure 2 our PureCube Fe-NTA magnetic beads presented themselves to be the best option for phosphopeptide enrichment. With our Fe-NTA MagBeads the most unique phosphopeptides (Fig. 2 A and C) were enriched with the highest efficiency (Figure 2 B).

Source: Leutert et al. (2019)
The best product for the best prize
Analyzing and comparing products from various manufacturers and suppliers can often prove to be a laborious task. The presence of varying concentrations and volumes across different suppliers can lead to confusion when trying to identify the optimal cost-benefit ratio. Recognizing these challenges, Cube Biotech has undertaken the effort to compile a comprehensive overview of prices for the frequently utilized phosphopeptide enrichment products (see Fig. 2 C). Our intention is to provide researchers with a clearer understanding of the prevailing pricing landscape in the market, facilitating informed decision-making.
Analyzing and comparing products from various manufacturers and suppliers can often prove to be a laborious task. The presence of varying concentrations and volumes across different suppliers can lead to confusion when trying to identify the optimal cost-benefit ratio. Recognizing these challenges, Cube Biotech has undertaken the effort to compile a comprehensive overview of prices for the frequently utilized phosphopeptide enrichment products (see Fig. 2 C). Our intention is to provide researchers with a clearer understanding of the prevailing pricing landscape in the market, facilitating informed decision-making.

FAQ
Can I get the datasheet for the Fe-NTA MagBeads?
What can I do with Fe-NTA beads?
Fe-NTA beads serve two purposes. First, they can be used as the matrix to bind and thus enrich phophopeptides. Second, they can be used similar to e.g Ni-NTA to purify His-tagged proteins via IMAC.
How does the phosphopeptide enrichment process work?
Can I enrich all phosphopeptides using Fe-NTA?
No, this is not possible. Not all phosphopeptides bind to Fe-NTA beads. As shown in figure 2 multiples bead types must be used to cover all phosphopeptides off a cell. Because of that we also offer Ti- Zr- and Al-NTA beads.