Corona Virus Proteins


Proteins
Update July 3rd 2020: We presented our coronavirus SPIKE protein research results at the German Corona Showcase! Click here to see the video of the whole presentaion.

The Essential Proteins for The COVID19 disease - For your research

COVID19 is (most likely) the year defining topic of 2020, Even beyond life sciences and medicine. Trying to find a working treatment for the disease is crucial and Cube Biotech contributes to the global research. As leading experts in the field of membrane protein stabilization and solubilization it is a logical step for us to provide you with aliquots of pure & full lenght ACE2 , SPIKE and TMPRSS2 protein in MSP and synthetic nanodiscs.
Corona Virus cell Infection Cycle Figure 1: Depiction of the SARS-CoV2 infection scheme.
First the surface protein of the Coronavirus called SPIKE is processed through a proteolytic cleavage by the cell surface serine protease TMPRSS2.
This brings the SPIKE protein into its active state in which it can interact with the cell surface receptor and metallocarboxypeptidase ACE2. This subsequently leads to the fusion on the virus membrane with the cell membrane similar to a vesicular import. This way the infection viral RNA genome enters the cell.
 
ProteinOriginal FunctionStatus of proteinInterested?
ACE2 Metallocarboxy peptidase on the cell's surface Full lenght membrane protein stabilized in nanodiscs
TMPRSS2 Serine protease located on the cell's surface Full lenght membrane protein stabilized in nanodiscs
SPIKE SARS-CoV2's surface protein Full lenght membrane protein stabilized in nanodiscs
 

SPIKE: SARS-CoV-2's key into our cells.

We offer the SPIKE protein solubilized in three different ways: Synthetic nanodiscs, detergent mycelles and MSP nanodiscs. Choose your favourite.

Alternatively choose our 3 in 1 set that contains all 3 options.
Product
SPIKE in Synthetic nanodiscs
 
SPIKE in Detergent Mycelles
 
SPIKE in MSP Nanodisc
 
3 in 1 SPIKE protein Set
Corona SPIKE protein
 
The spike protein (S protein) is a large type I transmembrane protein ranging from 1,160 amino acids for avian infectious bronchitis virus (IBV) and up to 1,400 amino acids for feline coronavirus (FCoV) (Figure 1). In addition, this protein is highly glycosylated as it contains 21 to 35 N-glycosylation sites2.
 

SPIKE - Full-Length & Complete by Cube Biotech

Right now, the lifescience market is flooded with coronavirus protein offers. But our own market analysis shows that only fragments or single domains can be obtained from other competitors. As experts for membrane proteins we successfully stabilized the full-lenght trimeric version of SARS-CoV-2's SPIKE protein.
 
Full-Length trimeric SPIKE protein
Figure 2: Overview of the current state of the SARS-CoV-2 SPIKE protein market. Cube Biotech is the only company that offers the full-length trimeric & active version. Therefore Cube Biotech comes the closest to the native SPIKE proteins structure.

 

ACE2 and TMPRSS2

ACE 2 receptor:
 
TMPRSS2:
ACE2 receptor Angiotensin-converting enzyme 2 (ACE2) is an enzyme attached to the outer surface (cell membranes) of cells in the lungs, arteries, heart, kidney, and intestines. It is a zinc containing metalloenzyme located on the surface of endothelial and other cells.1
 
TMPRSS2 Serine protease Transmembrane protease, serine 2 is an enzyme that in humans is encoded by the TMPRSS2 gene. Aside from the role in the SARS-CoV2 infection, its biological function remains unknown3.
 

Cube Biotech at the German Corona Showcase

We have presented our achievements in regards to the coronavirus SPIKE protein at the German Corona Showcase. We recorded the presentation for you. You can find the video in YouTube here:

References

  1. Turner A.J.,(2015) "Chapter 25 - ACE2 Cell Biology, Regulation, and Physiological Functions" Academic Press : Pages 185-189.
  2. Belouzard, S., Millet, J. K., Licitra, B. N., & Whittaker, G. R. (2012). Mechanisms of coronavirus cell entry mediated by the viral spike protein. Viruses, 4(6), 1011–1033. https://doi.org/10.3390/v4061011
  3. https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=7113, source: 16th April 2020 16.04.2020 4:21 pm CET
Corona Virus Proteins
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SPIKE in LMNG Mycelle, product image SARS CoV-2 spike protein in LMNG detergent
The surface protein SPIKE of SARS-CoV-2 stabilized in LMNG detergent mycelles. Full-Length and in native trimeric form!
Article number: 28702
Sales price: From €650.00 *
SARS CoV-2 spike protein set in LMNG detergent (100 µg), DIBMA Glycerol (25 µg) and nanodisc complex SARS CoV-2 spike protein set in LMNG detergent...
Cube Biotech offers SPIKE protein (SARS-CoV-2) stabilized in three different forms: Detergents; MSP and synthetic nanodiscs.
Article number: 28704
Sales price: €2,290.00 *
Product Image SPIKE protein in DIBMA SARS CoV-2 spike protein in DIBMA Glycerol
The surface protein SPIKE of SARS-CoV-2 stabilized in synthetic nanodisc. Full-Length and in native trimeric form!
Article number: 28706
Sales price: From €850.00 *
SPIKE in MSP nanodisc, product image SARS CoV-2 Spike protein nanodisc complex
The surface protein SPIKE of SARS-CoV-2 stabilized in MSP nanodisc. Full-Length and in native trimeric form!
Article number: 29301
Sales price: From €990.00 *
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