SARS-CoV-2 Spike Protein Nanodisc Complex

Order number: 29301

SPIKE in MSP nanodisc, schematic depiction

€1,327.00*

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Description

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers this intact form of SPIKE. Figure 1 highlights how this stabilization is achieved and provides insights into the overall structure of SPIKE.

Furthermore, Cube Biotech provides the following additional SARS-CoV-2 related products:

You can obtain SPIKE in MSP nanodiscs as a part of our SARS-CoV-2 Spike Protein Set.

SPIKE stabilized in a LMNG mycelle and depiction of its individual domains — **Figure 1:** Overview of SPIKE's domains and overall structure. Note that the protein is a homo-trimer. Using MSP nanodiscs that trimer remains intact.

Datasheets

Download SPIKE in MSP nanodisc Datasheet

Feature
Alternative names	SPIKE_SARS2 Spike glycoprotein
UniProt Number	P0DTC2
Protein class	Single span transmembrane protein
Organism	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence, explained	Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tags	C-terminal Rho1D4-tag
Expression Host	Hek293 Expi cells
Size	1286 amino acids (including Rho1D4-tag and linker) 142114 Da
Buffer composition	20 mM Hepes pH 7.5; 150 mM NaCl,
Stabilizing MSP nanodisc	MSP1D1-His, assembly with POPC lipids (Membrane scaffold protein based nanodisc; MSP1D1 = 25,3 kDa)
Purity	>98%, determined via SDS-PAGE (Figure 2 and 3)
Homogeneity	Native Gel, see Fig. 2 and 3
Purified via	PureCube Rho1D4 Agarose
Function	Host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Shipping conditions	Dry Ice
Storage conditions	–80°C. Avoid freeze-thaw cycles

Lab Results

SPIKE (wt) MSP nanodisc SDS-PAGE — **Figure 2:** SDS Page of purified SPIKE protein in MSP1D1 nanodiscs. The band that represents the MSP protein can also be seen.

MSP nanodisc SPIKE gel filtration — **Figure 3:** Gel filtration result of SPIKE in MSP nanodisc. The peak that represents spike is marked with a red arrow.

FAQ

Can I get the datasheet for the SPIKE (wt) protein in MSP nanodiscs?

Yes. Just click here to download it as a PDF File.

Can I infect myself with COVID-19 while working with this product? With the product being the source of the infection.

No, this is the isolated surface protein of the virus. Not a complete and harmful version of SARS-CoV-2.

Do you also offer other SPIKE variants / mutations?

Yes, we do! Look at THIS LIST.

Do you also offer other full-length membrane proteins?

Yes, we do. Just browse through our product protofolio. In case we do not have your desired membrane protein in our store, send a request to our protein services.