PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC based protein purification and protein enrichment methods. Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. By providing our coupling protocols, feel free to create any NTA purification resin you desire. PureCube NTA resin is also available with a bead diameter of 100 µm or even XL sized for higher FPLC flow rates or as NTA magnetic beads. Furthermore PureCube NTA agarose can also be obtained pre-filled into FPLC cartridges / columns.
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Features
Usage
For coupling of metal ions for corresponding purification and / or enrichment purposes
Specifity
Varies
Binding capacity
Depends on the loaded ion
Purity
Depends on the loaded ion
Bead Ligand
NTA
Bead size
40 μm
Matrix
7.5% cross-linked agarose
Recommended FPLC flow rates
0.5-2 mL/min
Maximal FPLC flow rate
6 mL/min
pH stability
2-14
Other stabilities
100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
