PureCube HiCap StrepTactin Agarose
Order number: 34101
Description
Our PureCube Strep-Tactin® agarose resin beads consist of 4% cross-linked agarose that is coupled to the Strep-Tactin® protein. They enable protein yields of up to 5 mg protein per ml resin.
They were developed for FPLC and batch spin-based protein purification. PureCube Strep-Tactin® agarose can also be purchased pre-packed in FPLC cartridges and columns Furthermore, PureCube Strep-Tactin® agarose XL resin is also available for higher FPLC flow rates or very viscous cell media. Alternatively Cube Biotech also offers PureCube Strep-Tactin® magnetic beads/MagBeads.
They were developed for FPLC and batch spin-based protein purification. PureCube Strep-Tactin® agarose can also be purchased pre-packed in FPLC cartridges and columns Furthermore, PureCube Strep-Tactin® agarose XL resin is also available for higher FPLC flow rates or very viscous cell media. Alternatively Cube Biotech also offers PureCube Strep-Tactin® magnetic beads/MagBeads.
Datasheets
| Feature | |
|---|---|
| Usage | Highly specific affinity purification of Strep-(WSHPQFEK)-tagged proteins |
| Specificity | Affinity to Strep-tagged (WSHPQFEK) proteins |
| Binding capacity | >5 mg/ml |
| Bead Ligand | Strep-Tactin® |
| Bead size | 100 μm |
| Matrix | 4% cross-linked agarose |
| Recommended flow rates | 0.25 - 1 ml/min |
| Max. flow rates | 6 ml/min |
| Filling quantity | Delivered as a 50 % suspension |
| Chemical stability | 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol |
| Short-term storage | In neutral buffer at 4°C |
| Long-term storage | In neutral buffer with 20% ethanol at 4°C |
| Required equipment |
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FAQ
Can I get the datasheet for the PureCube Strep-Tactin® Agarose?
Is Strep-Tactin® the same as Streptavidin?
No, they are not. Strep-Tactin® is a bioengineered protein, originally based on Streptavidin. However, Strep-Tactin® cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.
Can I regenerate & reuse your Strep-Tactin® beads?
Disclaimer
Strep-Tactin® and Strep-tag® are trademarks of IBA GmbH. PureCube Strep-Tactin® Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".