
- Order number: 34103
The term Strep®-tag refers to an eight amino acid long (WSHPQFEK) protein affinity tag that... more
Product information "PureCube HiCap StrepTactin Agarose"
Type: | Agarose |
Bead size: | 100 µm (Agarose) |
Affinity : | Strep-tag |
Purpose: | Protein Purification |
The term Strep®-tag refers to an eight amino acid long (WSHPQFEK) protein affinity tag that can be used for affinity chromatography based protein purification. It is based in the increadible specificity of the protein StrepTactin ® that interacts with the specific amino acid squence WSHPQFEK. Exploiting this incredible specificity maximum protein puritiy levels through affinity chromatography are possible.
Our PureCube StrepTactin agarose resin beads consists of 7.5% cross-linked agarose that are coupled to the StrepTactin protein. They enable protein yields of up to 5 mg protein per ml resin. They were developed for FPLC and batch spin based protein purification. PureCube StrepTactin® agarose can also be purchased pre-packed in cartridges and columns. Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
Our PureCube StrepTactin agarose resin beads consists of 7.5% cross-linked agarose that are coupled to the StrepTactin protein. They enable protein yields of up to 5 mg protein per ml resin. They were developed for FPLC and batch spin based protein purification. PureCube StrepTactin® agarose can also be purchased pre-packed in cartridges and columns. Alternatively Cube Biotech also offers PureCube StrepTactin® magnetic beads / MagBeads.
Features
Usage | Highly specific affinity purification of Strep®(WSHPQFEK)-tagged proteins |
Specifity | Affinity to Strep®-tagged proteins (WSHPQFEK) |
Binding capacity | 5 mg / ml resin |
Bead Ligand | StrepTactin ® |
Bead size | 100 μm |
Stability | 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, n-Octyl-beta-D-glucopyranoside, 0,2% n-Nonyl-beta-D-gluyopyranoside, 0,35% N-Decyl-beta-D-maltoside, 2% Lauryl-sacrosine, 0,1% SDS, 0,3% CHAPS, 1M Guanidine HCl, 1mM PMSF, 10% Ethanol, 5M NaCl, 2M (NH4)SO4, 1M CaCl2, 25% Glycerol |
Filling quantity | Delivered as a 50 % suspension |
Required equipment |
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