Run membrane protein purification on autopilot
Go from lysate to purified proteins in 2 hours with ready-to-run PlateX MP™ plates.
Qualify for early testing Watch on-demand webinar
Membrane protein purification should be easy!
But in practice, membrane proteins require special attention, especially if you need to preserve native protein properties for downstream assays.
Top 3 bottlenecks in membrane protein purification
Manual
handling
- Protocols are long
- It’s error-prone and can be user-dependent
- Purifying many samples manually is not scalable
Optimising
conditions
- Each protein behaves differently
- Ideal conditions cannot be predicted - screening is essential
- This adds time and complexity to the purification stage of a project
Preserving native
protein behaviour
- Solubilisation and stabilisation agents are key for native protein prep
- Traditional protocols remove native lipid environment with detergents
- Identifying best solubilisation agent takes time
PlateX MP™ plates – a tool for easier and faster membrane protein purification
PlateX MP™ plates are a ready-to-run product, designed for use with liquid handling robots. The workflow combines two experimental processes in a single run:
Screening
For optimal solubilisation agents that preserve native protein properties
Purification
Of membrane proteins that delivers analytical-grade proteins in only 2 hour
What do PlateX MP™ users say
Artem Evdokimov
CSO at Orogen Therapeutics
“Cube’s polymer-based workflow for automated membrane protein purification is a game-changing innovation. Having personally purified multiple GPCRs and ion channels using detergent micelles and protein nanodiscs, I was struck by the speed and simplicity of purifying membrane proteins directly from resuspended biomass into polymer nanodiscs in an essentially hands-off manner. The ability to screen multiple polymers in parallel often enables the rapid generation of functionally relevant protein samples on the first attempt.”
How do PlateX MP™ plates perform
To evaluate performance of PlateX MP™ plates, we purified multiple membrane proteins and assessed their yield, purity and stability. We show data for two GPCRs, a gap junction protein and an ion channel. We also evaluated the latter for suitability for downstream cryo-EM analysis.
See experimental data from a PlateX MP™ user
Watch an on-demand webinar to see how early adopters used PlateX MP™ plates in a fully automated workflow for purification of GCPR proteins.
Webinar recording
What do PlateX MP™ users need to do
The workflow includes 3 steps:
Step 1
Load the PlateX MP™ plate with cell lysate.
Step 2
Start the run on your liquid handling robot.
Step 3
Collect purified proteins in 2 hours.
How does the PlateX MP™ technology work
PlateX MP™ plates are pre-filled deep well plates that allow to screen for optimal solubilisation agents and purify membrane proteins, in a single run.
INTEGRA PROTOCOL DOWNLOAD ANALYTIK JENA PROTOCOL DOWNLOAD
PlateX MP™ plates are ready-to-run
The plates include all required components for the workflow:
- Pre-filled with a workflow mix of components for the entire protocol
- Lyophilised components, stable at room temperature
- Validated protocols for Integra and Jena Analytik liquid handling robots
The ready-to-run plate format eliminates manual steps so you can walkaway and let the robot run the whole workflow.
‘2 in 1 workflow mix’ for minimal hands-on time
The pre-loaded workflow mix includes all components for screening of optimal solubilisation agents (copolymers) while running the purification on your robot. The PlateX MP™ plates include:
- 8 copolymers for identification of optimal solubilisation agents
- Magnetic beads for affinity tag-based purification (3 options available)
- Components for all buffers already pre-loaded
This ‘2 in 1 workflow’ setup reduces time-to-purified protein by eliminating separate optimisation experiments and manual handling.
Synthetic copolymers for native-like protein properties
The PlateX MP™ plates include 8 synthetic copolymers from the Cubipol product range. Cubipols are next-generation copolymers that solubilise and stabilise membrane proteins in the form of nanodiscs.
- 8 Cubipol compounds for a full spectrum of copolymer properties
- Purified membrane proteins keep their native lipid environment
- Identify optimal copolymers for your protein in a single run
Copolymers preserve native membrane protein properties and are suitable for downstream assays such as drug screening or structural studies.
Why trust us
PlateX MP™ plate was developed by Cube Biotech’s scientists with extensive experience in purifying difficult membrane proteins. The product is optimised for membrane proteins and is truly unique, as it is built upon five Cube Biotech patented technologies, developed for easier and more effective protein purification workflows.
PlateX MP™ products
Choose the right product for your system depending on type of affinity tag and type of deep-well plate you require.
DYKDDDDK-/FLAG-tag
PlateX MP™ Axygen plates DYKDDDDK-/FLAG-tagPlateX MP™ INTEGRA plates DYKDDDDK-/FLAG-tag
