Glucagon-like peptide 1 receptor (GLP1R)

Order number: 70013

On Request

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Amount

Description

We are excited to introduce our off-the-shelf, full-length, active, and native Glucagon-like Peptide 1 Receptor (GLP1R). GLP1R is a critical G-protein-coupled receptor involved in glucose metabolism and insulin secretion, making it a key target in diabetes and metabolic research.

Our GLP1R protein has been solubilized and stabilized using Cube Biotech's NativeMP™ platform, ensuring it retains its native structure and full biological activity, making it ideal for a wide range of research applications.

Applications:

Screening/Validation
Biologics Development
Structure Determination
Small Molecule Drug Development

The protein delivered can be used for these specific applications. When ordering, please indicate which applications you intend to use the protein for in the contact form to ensure the best results.

For more details, explore our solubilization database.

copolymer vs detergents workflow — **Fig. 1:** Detergents create environments that lack native lipids, which can compromise protein function and stability. Copolymers, enable you to see and analyze these aspects within a native environment. They facilitate studying how your target interacts with its surrounding lipids, and allow assays to be conducted at body temperature.

Datasheets

Upon ordering, you will receive the corresponding datasheets and the Certificate of Analysis (CoA).

Feature
Alternative names	GLP1R
UniProt ID	P43220
Protein class	GPCR
Organism	Homo sapiens
Expression host	Insect (Trichoplusia ni)
Sequence, One-Letter Code	MKTIIALSYIFCLVFARPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLFLYIIYTVGYALSFSALVIASAILLGFRHLHCTRNYIHLNLFASFILRALSVFIKDAALKWMYSTAAQQHQWDGLLSYQDSLSCRLVFLLMQYCVAANYYWLLVEGVYLYTLLAFSVLSEQWIFRLYVSIGWGVPLLFVVPWGIVKYLYEDEGCWTRNSNMNYWLIIRLPILFAIGVNFLIFVRVICIVVSKLKANLMCKTDIKCRLAKSTLTLIPLLGTHEVIFAFVMDEHARGTLRFIKLFTELSFTSFQGLMVAILYCFVNNEVQLEFRKSWERWRLEHLHIQRDSSMKPLKCPTSSLSSGATAGSSMYTATCQASCSGGHHHHHHHHHHLEVLFQGPGSSGTETSQVAPA
Residues	24-463
Affinity tags	HA Signal peptide, His10-HRV3C-Rho1D4 (C-terminal)
Size (excluding additional elements)	440 amino acids, 51 kDa
Concentration	0.4 - 0.7 mg/ml
Purity	>90%, determined via SDS-PAGE
Purification process	2-step purification
Shipping	Shipment is carried out on dry-ice for temperature stability to ensure the integrity of the product.
Function	G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed:8405712, PubMed:8216285, PubMed:7517895, PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (PubMed:8405712, PubMed:8216285, PubMed:7517895, PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449). Plays a role in regulating insulin secretion in response to GLP-1 (By similarity). {ECO:0000250\|UniProtKB:O35659, ECO:0000269\|PubMed:19861722, ECO:0000269\|PubMed:26308095, ECO:0000269\|PubMed:27196125, ECO:0000269\|PubMed:28514449, ECO:0000269\|PubMed:7517895, ECO:0000269\|PubMed:8216285, ECO:0000269\|PubMed:8405712}.
PubMed ID	8405712, 8404634, 8216285, 7517895, 7843404, 14574404, 15489334, 9213353, 20869417, 21901419, 22412906, 18287102, 19861722, 26308095, 27196125, 28514449

Lab Results

GLP1R WT Eluate +DLS — **Figure 2:** Exemplary in-house SDS-Page, DLS, MST, and AS-MS data of GLP1R WT NativeMP™ copolymer screens. More in-depth data sets can be accessed upon project initiation.