Toll-like receptor 5 (TLR5, TIL3)

Order number: 70071

€14,800.00*

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Description

We are pleased to introduce our off-the-shelf, full-length, active, and native Toll-like Receptor 5 (TLR5, TIL3). TLR5 is a vital component of the innate immune system, responsible for recognizing bacterial flagellin and triggering immune responses. Its role is significant in various diseases, including inflammatory bowel disease, rheumatoid arthritis, bone loss, and certain cancers, where immune dysregulation can lead to chronic inflammation or tumor progression.

Our TLR5 protein has been solubilized and stabilized using Cube Biotech's NativeMP™ platform, ensuring that it maintains its native structure and full biological activity. This makes it an excellent choice for diverse research applications.

Applications:

Screening/Validation
Biologics Development
Structure Determination
Small Molecule Drug Development

The protein delivered can be utilized for these specific applications. When ordering, please specify which applications you intend to use the protein for in the contact form to ensure the best results.

For more details, explore our solubilization database.

Cube’s NativeMP™ Platform enables high-resolution structural studies with compounds and allows assays to be conducted at body temperature, saving valuable time in your research efforts.

copolymer vs detergents workflow — **Fig. 1:** Detergents create environments that lack native lipids, which can compromise protein function and stability. Copolymers, enable you to see and analyze these aspects within a native environment. They facilitate studying how your target interacts with its surrounding lipids, and allow assays to be conducted at body temperature.

Datasheets

Upon ordering, you will receive the corresponding datasheets and the Certificate of Analysis (CoA).

Feature
Alternative names	TLR5, TIL3
UniProt Number	O60602
Protein class	Receptor
Organism	Homo sapiens
Sequence, One-Letter Code	MGDHLDLLLGVVLMAGPVFGIPSCSFDGRIAFYRFCNLTQVPQVLNTTERLLLSFNYIRTVTASSFPFLEQLQLLELGSQYTPLTIDKEAFRNLPNLRILDLGSSKIYFLHPDAFQGLFHLFELRLYFCGLSDAVLKDGYFRNLKALTRLDLSKNQIRSLYLHPSFGKLNSLKSIDFSSNQIFLVCEHELEPLQGKTLSFFSLAANSLYSRVSVDWGKCMNPFRNMVLEILDVSGNGWTVDITGNFSNAISKSQAFSLILAHHIMGAGFGFHNIKDPDQNTFAGLARSSVRHLDLSHGFVFSLNSRVFETLKDLKVLNLAYNKINKIADEAFYGLDNLQVLNLSYNLLGELYSSNFYGLPKVAYIDLQKNHIAIIQDQTFKFLEKLQTLDLRDNALTTIHFIPSIPDIFLSGNKLVTLPKINLTANLIHLSENRLENLDILYFLLRVPHLQILILNQNRFSSCSGDQTPSENPSLEQLFLGENMLQLAWETELCWDVFEGLSHLQVLYLNHNYLNSLPPGVFSHLTALRGLSLNSNRLTVLSHNDLPANLEILDISRNQLLAPNPDVFVSLSVLDITHNKFICECELSTFINWLNHTNVTIAGPPADIYCVYPDSFSGVSLFSLSTEGCDEEEVLKSLKFSLFIVCTVTLTLFLMTILTVTKFRGFCFICYKTAQRLVFKDHPQGTEPDMYKYDAYLCFSSKDFTWVQNALLKHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLEAFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDLQDVGWFLHKLSQQILKKEKEKKKDNNIPLQTVATISGSSGTETSQVAPA
Affinity tags	Rho1D4-tag (C-terminal)
Size (excluding additional elements)	858 amino acids, 98 kDa
Concentration	0.4 - 0.7 mg/ml (forms Homodimers)
Purity	>90%, determined via SDS-PAGE
Purification process	2-step purification
Shipping	Shipment is carried out on dry-ice for temperature stability to ensure the integrity of the product.
Function	Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response (PubMed:11323673, PubMed:18490781). Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota (PubMed:29934223). Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response (PubMed:20855887, PubMed:11489966). Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity). {ECO:0000250\|UniProtKB:Q9JLF7, ECO:0000269\|PubMed:11323673, ECO:0000269\|PubMed:11489966, ECO:0000269\|PubMed:18490781, ECO:0000269\|PubMed:20855887, ECO:0000269\|PubMed:29934223}.
PubMed ID	9596645, 18810425, 19179655, 19924287, 16710414, 15489334, 9435236, 15340161, 11489966, 11323673, 16027372, 17157808, 17442957, 18490781, 20855887, 23447684, 24778236, 29934223, 22173220, 14623910

Lab Results

TLR5 Eluate +DLS — **Figure 2:** Exemplary in-house SDS-Page and DLS data of TLR5 NativeMP™ copolymer screens. More in-depth data sets can be accessed upon project initiation.