SARS-CoV-2 Spike Protein in DIBMA Glycerol
Order number: 28707
Description
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers this intact form of SPIKE. Figure 1 highlights how this stabilization is achieved and provides insights into the overall structure of SPIKE.
Furthermore, Cube Biotech provides the following additional SARS-CoV-2 related products:
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers this intact form of SPIKE. Figure 1 highlights how this stabilization is achieved and provides insights into the overall structure of SPIKE.
Furthermore, Cube Biotech provides the following additional SARS-CoV-2 related products:
- Wild-type SPIKE stabilized in different ways
- SPIKE from SARS-CoV-2 mutants (e.g. Omicron)
- Anti-SPIKE antibodies.
Datasheets
| Feature | |
|---|---|
| Alternative names | SPIKE_SARS2 Spike glycoprotein |
| UniProt Number | P0DTC2 |
| Protein class | Single span transmembrane protein |
| Organism | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
| Sequence, explained | Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence |
| Affinity tags | C-terminal Rho1D4-tag |
| Expression Host | Hek293 Expi cells |
| Size | 1286 amino acids (including Rho1D4-tag and linker) 142114 Da |
| Buffer composition | 20 mM Hepes pH 7.5; 150 mM NaCl, buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid) |
| Stabilization agent | DIBMA Glycerol |
| Purity | >98%, determined via SDS-PAGE (Figure 2 A and B) |
| Homogeneity | Native Gel, see Fig. 2 C |
| Purified via | PureCube Rho1D4 Agarose |
| Function | Host cell surface receptor binding; fusion of virus membrane with host endosome membrane |
| Shipping conditions | Dry Ice |
| Storage conditions | –80°C. Avoid freeze-thaw cycles |
Lab Results
FAQ
Can I get the datasheet for the SPIKE (wt) in DIBMA Glycerol protein?
Can I infect myself with COVID-19 while working with this product? With the product being the source of the infection.
No, this is the isolated surface protein of the virus. Not a complete and harmful version of SARS-CoV-2.
Do you also offer other SPIKE variants / mutations?
Yes, we do! Look at THIS LIST.
Do you also offer other full-length membrane proteins?
Yes, we do. Just browse through our product protofolio. In case we do not have your desired membrane protein in our store, send a request to our protein services.