SARS-CoV-2 Spike Protein in DIBMA Glycerol

Order number: 28707

€3,213.00*

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Description

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers this intact form of SPIKE. Figure 1 highlights how this stabilization is achieved and provides insights into the overall structure of SPIKE.

Furthermore, Cube Biotech provides the following additional SARS-CoV-2 related products: You can obtain SPIKE in DIBMA Glycerol as a part of our SARS-CoV-2 Spike Protein Set.
SPIKE stabilized in a DIBMA copolymer nanodisc and depiction of its individual domains
Figure 1: Overview of SPIKE's domains and overall structure. Note that the protein is a homo-trimer. Using DIBMA Glycerol that trimer remains intact.
Feature
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt Number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence, explained Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tags C-terminal Rho1D4-tag
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4-tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl, buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid)
Stabilization agent DIBMA Glycerol
Purity >98%, determined via SDS-PAGE (Figure 2 A and B)
Homogeneity Native Gel, see Fig. 2 C
Purified via PureCube Rho1D4 Agarose
Function Host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Shipping conditions Dry Ice
Storage conditions –80°C. Avoid freeze-thaw cycles

Lab Results

SPIKE protein in DIBMA nanodisc
Figure 2: Data from our lab regarding SPIKE stabilized and purified in synthetic nanodiscs (Dibma Glycerol). A: SDS-PAGE of SPIKE in synthetic nanodisc. B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A" C: Native protein Gel of purified and stabilized SPIKE in synthetic copolymer nanodisc.

FAQ

Can I get the datasheet for the SPIKE (wt) in DIBMA Glycerol protein?

Can I infect myself with COVID-19 while working with this product? With the product being the source of the infection.

No, this is the isolated surface protein of the virus. Not a complete and harmful version of SARS-CoV-2.

Do you also offer other SPIKE variants / mutations?

Yes, we do! Look at THIS LIST.

Do you also offer other full-length membrane proteins?

Yes, we do. Just browse through our product protofolio. In case we do not have your desired membrane protein in our store, send a request to our protein services.