SARS-CoV-2 Spike Protein in LMNG Detergent

Order number: 28703

SPIKE in LMNG mycelle, schematic depiction

€2,812.00*

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Description

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers this intact form of SPIKE. Figure 1 highlights how this stabilization is achieved and provides insights into the overall structure of SPIKE.

Furthermore, Cube Biotech provides the following additional SARS-CoV-2 related products:

You can obtain SPIKE in LMNG as a part of our SARS-CoV-2 Spike Protein Set.

SPIKE stabilized in a LMNG mycelle and depiction of its individual domains — **Figure 1:** Overview of SPIKE's domains and overall structure. Note that the protein is a homo-trimer. Using LMNG detergent that trimer remains intact.

Datasheets

Download SPIKE in LMNG mycelle Datasheet

Feature
Alternative names	SPIKE_SARS2 Spike glycoprotein
UniProt Number	P0DTC2
Protein class	Single span transmembrane protein
Organism	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence, explained	Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tags	C-terminal Rho1D4-tag
Expression Host	Hek293 Expi cells
Size	1286 amino acids (including Rho1D4-tag and linker) 142114 Da
Buffer composition	20 mM Hepes pH 7.5; 150 mM NaCl, 0.01 % LMNG
Purity	>98%, determined via SDS-PAGE (Figure 2 A and B)
Homogeneity	Native Gel, see Fig. 2 C
Purified via	PureCube Rho1D4 Agarose
Function	Host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Shipping conditions	Dry Ice
Storage conditions	–80°C. Avoid freeze-thaw cycles

Citations

Year	Author
2023	Kerkhoff Y.
2023	Kuhn C.C.
2023	Kuhn C.C., Basnet N., Bodakuntla S., Alzarez-Brecht P., Nichols S., Martinez-Sanchez A., Agostini L., Soh Y-M., Takagi J., Biertümpfel C., Mizuno N.
2024	Roodink I., van Erp M., Li A., Potter S., van Duijinhoven S.M.J., Smits M., Kuipers A.J., Kazemier B., Berkeveld B., van Geffen E., de Vries B.S., Rijbroek D., Boers B., Meurs S., Hemrika W., Thom A., Duplantis B.N., Romijn R.A., Houser J.S., Bath J.L., Yasmina N. Abdiche

Lab Results

SPIKE (wt) SDS-PAGE — **Figure 2:** Data from our lab regarding SPIKE stabilized in a detergent mycelle. A: SDS-Page of purified SPIKE in detergent mycelle. B: Western blot of Rho1d4-tagged SPIKE, originated from the SDS Page seen in "A". C: Native protein Gel of purified and stabilized SPIKE in detergent mycelles.

FAQ

Can I get the datasheet for the SPIKE (wt) protein?

Yes. Just click here to download it as a PDF File.

Can I infect myself with COVID-19 while working with this product? With the product being the source of the infection.

No, this is the isolated surface protein of the virus. Not a complete and harmful version of SARS-CoV-2.

Do you also offer other SPIKE variants / mutations?

Yes, we do! Look at THIS LIST.

Do you also offer other full-length membrane proteins?

Yes, we do. Just browse through our product protofolio. In case we do not have your desired membrane protein in our store, send a request to our protein services.