Tumor necrosis factor receptor superfamily member 6 (FAS, APT1, FAS1, TNFRSF6)

Order number: 70081

€12,800.00*

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Amount

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Description

We are excited to introduce our off-the-shelf, full-length, active, and native Tumor Necrosis Factor Receptor Superfamily Member 6, commonly known as FAS. This essential protein forms the death-inducing signaling complex (DISC) upon ligand binding, which can trigger apoptosis and is associated with cancer. FAS plays a critical role in the immune system, making it a promising target for preclinical studies and assay development.

Our FAS protein has been solubilized and stabilized using our NativeMP™ platform, ensuring it retains its native structure and full biological activity, making it ideal for various research applications.

Applications:

Screening/Validation
Biologics Development
Structure Determination
Small Molecule Drug Development

The protein delivered can be used for these specific applications. When ordering, please indicate which applications you intend to use the protein for in the contact form to ensure the best results.

For more details, explore our solubilization database.

copolymer vs detergents workflow — **Fig. 1:** Detergents create environments that lack native lipids, which can compromise protein function and stability. Copolymers, enable you to see and analyze these aspects within a native environment. They facilitate studying how your target interacts with its surrounding lipids, and allow assays to be conducted at body temperature.

Datasheets

Upon ordering, you will receive the corresponding datasheets and the Certificate of Analysis (CoA).

Feature
Alternative names	FAS, APT1, FAS1, TNFRSF6
UniProt Number	P25445
Protein class	Receptor
Original host	Homo sapiens
Expression system	HEK293
Sequence, One-Letter Code	MLGIWTLLPLVLTSVARLSSKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTQEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLVGSSGTETSQVAPA
Affinity tags	Rho1D4-tag (C-terminal)
Size (excluding additional elements)	335 amino acids, 38 kDa (forms Homotrimers)
Ligand available	FASL
Concentration	0.4 - 0.7 mg/ml
Purity	>90%, determined via SDS-PAGE
Purification process	2-step purification
Shipping	Shipment is carried out on dry-ice for temperature stability to ensure the integrity of the product.
Function	Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). {ECO:0000269\|PubMed:19118384, ECO:0000269\|PubMed:7533181}.
PubMed ID	1713127, 1375228, 7575433, 7533181, 8598453, 8648105, 17336828, 14702039, 15164054, 15489334, 7538908, 10542291, 10535980, 14759258, 15465831, 18846110, 18691976, 18669648, 19159218, 21109225, 20068231, 21269460, 22171320, 23955153, 24275569, 25301068, 30979585, 8967952, 19118384, 24914971, 7540117, 8929361, 9028321, 9028957, 9322534, 9787134, 9821419, 10090885, 10515860, 10340403, 9927496, 10620127, 11418480, 20935634

Lab Results

FAS Eluate +DLS — **Figure 2:** Exemplary in-house SDS-Page and DLS data of FAS NativeMP™ copolymer screens. More in-depth data sets can be accessed upon project initiation.