Glyco-Cubipol

Order number: 18781

€492.00*

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Description

Welcome the new addition to our NativeMP™ Platform: The Cubipol Series.

The proprietary Cubipol backbone, developed in-house, condenses the positive effects of previously available backbones like SMA, DIBMA, and UltrasoluteTM Amphipol. This new development delivers a new multimodal copolymer backbone, making the Cubipol series the first commercially available next-generation copolymer with 4 available off-the-shelf modified variants.
It joins the Next Generation Copolymers AASTY and Ultrasolute™ Amphipol, making solubilization and stabilization of membrane proteins a simpler and more effective endeavor than ever before.
Screening is key to discovering the best copolymer to stabilize your membrane protein target. Try the copolymers individually or save time by screening once and identifying the best stabilizing copolymers for your specific application with our NativeMP™ Screening Kit.

Glyco-Cubipol

This modification of the Cubipol backbone is glucamine-based and is the derivate with the lowest charge of the entire Cubipol Series. This copolymer is well suited for charge sensitive downstream applications.
Feature
State Lyophilized Powder,
to be solved with water
Full Name Glyco-Cubipol
Buffer HEPES
pH after solving 7.5
pH Stability of Copolymer 6.0-10
Absorbance at 280nm N/A
Molecular Weight 12.2 kDA
Ca2+ tolerance 20 mM
Mg2+ tolerance 40 mM
Solubility > 10% (H2O)
Polymerization Type Free-Radical
Shipping Temperature Ambient Temparature
Storage of lyophilized copolymer -20°C for several years
Storage of dissolved copolymer 2-8°C for several days

Lab Results

Hydrpnamic Diameter of Cubipol Nanodiscs
Fig. 1: Hydrodynamic size of Cubipol-DMPC nanodiscs formed with different polymers. Nanodisc size was measured in 150 mM NaCl, 20 mM HEPES at pH 7.5 and a Polymer/DMPC weigth ratio of 3/1.
Wavelenght quantification measurements for Cubipol Nanodiscs
Fig. 2: UV-VIS Extinction measurements of all four Cubipols ranging from wavelength 200-400 nm. The only extinction peak can be measured at around 225nm.
Cubipol Zeta-Potential
Fig. 3: ζ-potential of DMPC nanodiscs formed with different Cubipol copolymers. All four reveal negative net charge. With Glyco-Cubipol being the closest to neutrality at around -15 mV. Meanwhile unmodified Cubipol has the most negative net charge at -25 mV.

Video

Watch this video and let Dr. Philipp Hanisch explain why you need to work with our NativeMP™ Copolymer Nanodiscs

Disclaimer

Patent Pending
The product described above has been developed and produced by Cube Biotech GmbH. Our products are intended for molecular applications. These products are not intended for diagnosis, prevention or treatment of a disease.