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NativeMP™ Screening Kit (30x1x50mg)

Order number: 18298
NativeMP™ Screening Kit (30x1x50mg)

€990.00*

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Description

Over 10 years of experience and expertise have culminated in the NativeMP™ copolymer technology featured in this screening kit. Achieve rapid solubilization and stabilization of membrane proteins in a single, detergent-free step.
Membrane proteins are notoriously difficult to stabilize while keeping their structural activity intact. With copolymers, this challenge is tackled by stabilizing the protein together with its native lipid environment. To ensure that you find the best copolymer to suit the unique needs of your membrane protein of interest, we have created a comprehensive selection in this kit. Contact us to learn if it’s suitable for your purposes!

Screening is key

Profit from 30 different copolymers derived from five main backbones: SMA, DIBMA, AASTY, Ultrasolute™ Amphipol and our newest innovation Cubipol. Do a screening to find the optimal copolymer for your membrane protein and save time on optimization processes.

Benefits

  • Convenient Process: Copolymers allow for a single-step solubilization and stabilization directly from the cell.
  • Temperature Stability: Experiments can be carried out at room temperature and up to 37°C.
  • Optimized Stabilization: Preserves the native lipid environment of the membrane protein, retaining its functional stability and avoiding disintegration even of large membrane protein complexes.
  • Faster Process: NativeMP™ Technology is 100% detergent-free. Forget about preparing different buffers and constantly having to add detergents to your solution. With copolymers, you avoid that and save valuable lab time.
  • Application compatibility: Our copolymer technology is compatible with many sensitive downstream applications like phage display, immobilization via SPR, ELISA, and more.
Contact us to learn if it’s suitable for your purposes!

Datasheets

Table 1: Shared Features

This table lists the features that each of the 30 copolymers share.
Feature  
Use Solubilization & stabilization of membrane proteins
Buffer HEPES
pH (upon solving in dH2O) 7.5
State upon delivery Lyophilized powder
Recommended concentration for use 1% - 5%
Shipping Temperature Room temperature
Long-term Storage (lyophilized copolymer) -20°C for several years
Short-term Storage (dissolved copolymer) 2°C - 8°C for several days


Table 2: Included SMALP Products

Feature SMALP 140 SMALP 140-I SMALP 200 SMALP 300 Sulfo-SMA
Molecular weight 5 kDa 5 kDa 6.5 kDa 10 kDa 8 kDa
Polymerization Type Free-Radical Free-Radical Free-Radical Free-Radical RAFT
Divalent Cationic Tolerance < 5 mM < 100 mM < 5 mM < 5 mM > 100 mM
Absorption at 280nm Yes Yes Yes Yes Yes
Solubility in dH2O 45% 38% 40% 35% > 10 %


Table 3: Included SMALP BZ Products

Feature SMALP BZ25 SMALP BZ30 SMALP BZ35 SMALP BZ40
Molecular weight 5.5 kDa 5.5 kDa 5.5 kDa 5.5 kDa
Polymerization Type RAFT RAFT RAFT RAFT
Divalent Cationic Tolerance < 5 mM < 5 mM < 5 mM < 5 mM
Absorption at 280nm Yes Yes Yes Yes
Solubility in dH2O > 10 % > 10 % > 10 % > 10 %


Table 4: Included DIBMA Products

Feature DIBMA 6 DIBMA 10 DIBMA 12 DIBMA Glycerol DIBMA Glucosamine Glyco-DIBMA Sulfo-DIBMA
Molecular weight 6 kDa 10 kDa 12 kDa 10 kDa 10 kDa 10 kDa 15.7 kDa
Polymerization Type RAFT Free-Radical Free-Radical Free-Radical Free-Radical Free-Radical RAFT
Divalent Cationic Tolerance < 50 mM < 5 mM < 5 mM < 50 mM < 50 mM < 4 mM > 100 mM
Absorption at 280nm No No No No No No No
Solubility in dH2O > 10 % > 10 % > 10 % > 10 % > 10 % > 10 % > 10 %


Table 5: Included AASTY Products

Feature AASTY 6-45 AASTY 6-50 AASTY 6-55 AASTY 11-45 AASTY 11-50 AASTY 11-55
Molecular weight 5-6 kDa 5-6 kDa 5-6 kDa 10-11 kDa 10-11 kDa 10-11 kDa
Polymerization Type RAFT RAFT RAFT RAFT RAFT RAFT
Divalent Cationic Tolerance < 6 mM < 6 mM < 6 mM < 6 mM < 6 mM < 6 mM
Absorption at 280nm Yes Yes Yes Yes Yes Yes
Solubility in dH2O > 10 % > 10 % > 10 % > 10 % > 10 % > 10 %


Table 6: Included Amphipol Products

Feature Ultrasolute™ Amphipol 17 Ultrasolute™ Amphipol 18
Molecular weight 7.3 kDa 7.3 kDa
Polymerization Type Free-Radical Free-Radical
Divalent Cationic Tolerance < 10 mM < 25 mM
Absorption at 280nm No No
Solubility in dH2O > 10 % > 10 %


Table 7: Included Cubipol Products

Feature Cubipol Cubipol Glycerol Sulfo-Cubipol Sulfo-Cubipol Lite Sulfo-Cubipol Medium Glyco-Cubipol
Molecular weight 7.2 kDa 10.7 kDa 13.7 kDa 9 kDa 11.7 kDa 12.2 kDa
Polymerization Type Free-Radical Free-Radical Free-Radical Free-Radical Free-Radical Free-Radical
Divalent Cationic Tolerance < 40 mM < 20 mM< 100 mM
 < 40 mM
< 40 mM
< 40 mM
Absorption at 280nm No No No No No N/A
Solubility in dH2O > 10 %
 > 10 %
 > 10 %
 > 10 %
> 10 %
> 10 %
pH Stability Range 4.5 - 10 6 - 10
 5.5 - 10 5.5 - 10
5.5 - 10
6 - 10


Lab Results

Fig. 1: Side-by-side comparison of the two most prominent stabilization techniques for membrane proteins. Copolymers solubilize and stabilize in a single step and preserve the membrane protein in its native environment. Detergents are added at several points during the stabilization process and stabilize the membrane protein without lipids in a detergent micelle.
Fig. 2: Screening of an ion channel at 4°C and 23°C with NativeMP copolymers.Classic copolymers include SMA and DIBMA. Next generation copolymers include AASTY, Ultrasolute Amphipol and Cubipol. Protein of interest was purified at 4°C/23°C from solubilizate via Rho-MagBeads, eluates were analyzed via SDS-Page and Western Blot. Working at 23°C significantly boosts the elution quantity (up to 200%) compared to 4°C.

Disclaimer

Our products are intended for molecular applications and are not intended for diagnosis, prevention, or treatment of disease.