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DIBMA 6 Biotin

Order number: 18771
DIBMA 6 Biotin
DIBMA 6 Biotin
DIBMA 6 Biotin
DIBMA 6 Biotin

€810.00*

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Description

The new DIBMA 6 is not only smaller than its predecessors (DIBMA 10 and DIBMA 12) but together with Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and lower polydispersity, meaning that if you need your copolymers to all have a similar size this is the copolymer to choose.

DIBMA 6 Biotin

This first-of-its-kind, functionalized version of our DIBMA 6 is tagged with biotin, enabling tag-free solubilization and stabilization of your target protein. There is no protein-labeling required, and the accesibility to the ecto- and cytoplasmatic domain is preserved.
This functionalized copolymer is particularly suited for downstream applications that require copolymer stabilization without affecting the protein. These include drug screening, high-throughput processes and assay development.

More applications for biotin-tagged copolymers:
  • Immobilization via SPR, ELISA, etc.
  • Sorting (FACS)
  • Quantification
  • Identification
  • Localization (Electron Microscopy)

Find more of our biotinylated copolymers included in the  NativeMP™ Biotin Kit. 

If you’re interested in having this functionalization applied to a different copolymer backbone, get in touch with us and we’ll get that project running for you: Contact us

Datasheets

Feature
State Lyophilized Powder, to be solved with water
Full Name Diisobutylene Maleic Acid Biotin in 50 mM HEPES, pH 7.5
Buffer HEPES
pH after solving 7.5
pH Stability of Copolymer 5.0-10
Absorbance at 280nm N/A
Molecular Weight 6 kDA
Ca2+ tolerance 30 mM
Mg2+ tolerance 40 mM
Solubility > 10% (H2O)
Polymerization Type RAFT
Shipping Temperature Ambient Temparature
Storage of lyophilized copolymer -20°C for several years
Storage of dissolved copolymer 2-8°C for several days

Lab Results

Molecular Size Distribution of DIBMA 6 Nanodiscs
Fig. 1: Size distribution of DIBMA 6 copolymers synthesized via RAFT compared to other copolymers synthesized via classical methods. Shown is the reduction in both monomer size and lower polydispersity for RAFT synthesized DIBMA 6.
DIBMA Variants Comparison
Fig. 2: Rho-tagged membrane protein has been solubilized and stabilized from celll membrane in one step at 23°C with an array of DIBMA copolymer variants including the new DIBMA 6 (here without biotin functionalization). Protein of interest was purified at 23°C from solubilizate via Rho-Magbeads, eluates were analysed via SDS-Page and Western-blot.
Fig. 3: Refractive Index overlay of Streptavidin and Streptavidin + DIBMA 6 Biotin (1:3) in 150 mM NaCl + 20 mM HEPES, pH 7.5, 30°C, 0.5 ml/min; Superdex 75 10/300.

Disclaimer

Our products are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.