PureCube Epoxy Activated Agarose
Order number: 50710
Description
PureCube Epoxy Agarose resin is your best option to couple biomolecules that carry free-standing amine or thiol groups. The resulting Amide or Thioether bond between the Epoxy Agarose beads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube Epoxy Activated Agarose is also available as magnetic beads in case you prefer this method, or as XL agarose resin if you need higher flow rates for your downstream application.
| Feature | |
|---|---|
| Usage | Coupling proteins to active Epoxy Groups |
| Specificity | Exposed amine (NH2) or thiol (SH) groups on biomolecule |
| Bead Ligand | Chemically active Epoxy groups |
| Epoxy Group density | 20 µmol/ml |
| Bead size | 40 μm |
| Filling quantity | Delivered as a 50 % suspension |
| Storage Buffer | 100% isopropanol |
| Matrix | 7.5% cross-linked agarose |
| Short Term Storage | In equilibration buffer (see protocol) at 4 °C |
| Long Term Storage | Best lyophilized, or in 100% isopropanol at 4°C |
| Shelf life | If properly stored - 6 months upon delivery, unopened |
| Chemical Structure |  |
Citations
| Coupled biomolecule | Year | Author |
|---|---|---|
| Artificial Silicon Carbon Enzyme | 2023 | Gallus S., Esther M., Weber A.J., Peng M., Niemeyer C.M., Rabe K.S. |
| l-lactate oxidase | 2024 | Luley-Goedl C., Bruni M., Nidetzky B. |
FAQ
Can I get the datasheet for the Epoxy Activated Agarose?
What can I do with these beads?
The beads carry reactive Epoxy groups on their surface. These can be used to bind all sorts of biomolecules if they carry free amine (NH2) or thiol (SH) groups. The subsequent use of the beads depends on what biomolecule was coupled.
How does the coupling work?
How long can I store the Epoxy Agarose?
The reactive properties of the Epoxy beads are very potent. But therefore, they degrade over time. We can safely say that upon delivery the beads last for up to 6 months. If properly stored of course.