His Affinity Resins & MagBeads


Purification Resins
The polyhistidine tag / Poly-His-tag is the most widely used protein affinity tag. Due to its small size of only six amino acids it rarely interferes with the protein's function. Additionally its low immunogenicity and versatility under native and denaturing conditions are hightlighting this protein tag among others. The underlying purification principle of the His-tag is an interaction between metal ions and the imidazole ring of histidine. CLICK HERE to learn more.

Cube Biotech's His tag purification products:

Coupled metal IonLigand
  NTA IDA INDIGO
Nickel / Ni2+ Ni-NTA products Ni-IDA products Ni-INDIGO products
Cobalt Co-NTA products  
Zinc Zn-NTA products  
Copper Cu-NTA products  
None
For Custom Metal Ions
NTA products IDA products  

To know about His-tag purification resins and magnetic beads:

Choosing the correct type of His-tag purification resin or magnetic bead is based on three factors:
  1. The Ligand: This is the molecule to which the metal ion is coupled in a chelator complex (see Figure 1).
    Cube Biotech offers three different ligands.
    • NTA: Nitrilotriacetic acid is the most commonly used ligand. It's chelator complex with Nickel is depited in figure 1.
    • IDA: Iminodiacetic acid is the second most commonly used one. CLICK HERE to learn the differences between IDA and NTA.
    • INDIGO:This ligand was developped here at Cube Biotech. Among its advantages is a highly increased DTT and EDTA tolerance. CLICK HERE to know more.

  2. The coupled metal Ion: Depending on which metal ion is coupled to the ligand the affinity and specificty of the His-tag purification changes. Rule of thumb: The higher the protein yield, the less specific the purification gets. Figure 2 gives an overlook one the affinty/specificty ratio of the most commonly used metal ions for His-tag purification.

  3. The size of the beads: The bigger the used beads are in diameter the higher the flow rate for FPLC purification. However having a slower flow rate usually increases the protein yield. We offer bead diameters of 30 µm, 40µm, 100 µm and XL sized (~400 µm). Further sizes on request available. See figure 3 for reference.

His-tag affinity resins and MagBeads in detail:

NTA chelator complex Figure 1: Schematic depiction of a chelator complex conisting of NTA and a Nickel2+. This Nickel Ion binds to two imidazol rings of a His-tag that has been added to a protein.
His tag affinity and specificity Figure 2: Overview on the most commonly used metal ions for His-tag protein purification. Copper has the highest affinity, while in turn having the lowest specificity. Cobalt marks the other end of the spectrum. Nickel, the most commonly used metal tions has a good balance between affinity and specificity.
 
Agarose bead size comparison Figure 3: Our commonly provided bead sizes in compairson. The smallest beads are magnetic beads with a 30 µm diameter and agarose resin with 40 µm. Our XL sized beads with 400 to 500 µm diameter are marking the other end of the spectrum. Sizes above that are depicted but have to be ordered on request.
 
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His Affinity Resins & MagBeads
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PureCube 100 INDIGO Ni-Agarose PureCube 100 INDIGO Ni-Agarose
Cube Biotech's exclusive INDIGO-Ni agarose resin for His Tag protein purification. Up to 100 mg protein / 1 ml resin.
Article number: 75103
Sales price: From €112.00 *
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Ni-NTA Agarose resin for His Tag protein purification. Ø 40 µm bead diameter. Great protein yield. For batch spin & FPLC!
Article number: 31103
Sales price: From €103.00 *
PureCube 100 Compact Cartridge Ni-INDIGO PureCube 100 Compact Cartridge Ni-INDIGO
Our exclusive & high quality INDIGO-Ni agarose resin pre-filled in COMPACT FPLC Cartridges / Columns for His-tagged proteins!
Article number: 75302
Sales price: From €52.00 *
PureCube Ni-NTA MagBeads PureCube Ni-NTA MagBeads
Ni-NTA magnetic beads for His Tag protein purification. Ø 30 µm bead diameter. Great protein yield. 25% settled beads!
Article number: 31201
Sales price: From €65.00 *
PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
25% concentrated INDIGO-Ni agarose for His-tag protein purification. EDTA and DTT stable. ~100% purity for your His-tagged protein.
Article number: 75201
Sales price: From €72.00 *
PureCube Co-NTA Agarose PureCube Co-NTA Agarose
Co-NTA Agarose resin for His-tag protein purification. Ø 40 µm bead diameter. Great protein purity. For batch spin & FPLC!
Article number: 31403
Sales price: From €111.00 *
PureCube Ni-NTA Agarose XL PureCube Ni-NTA Agarose XL
XL Sized Ni-NTA Agarose resin beads for His Tag protein purification. | ~Ø 400 µm bead diameter.| For viscous cell lystates.
Article number: 55103
Sales price: From €140.00 *
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Ni-NTA Agarose resin for His-tag protein purification. Ø 100 µm bead diameter. Great protein yield. For batch spin & FPLC!
Article number: 74103
Sales price: From €112.00 *
PureCube 100 INDIGO Ni-Cartridge PureCube 100 INDIGO Ni-Cartridge
Our exclusive & high quality INDIGO-Ni agarose resin pre-filled in FPLC Cartridges / Columns for your His Tag purifications!
Article number: 75301
Sales price: From €50.00 *
HisCube - Ni-INDIGO His-Tag Protein Purification MIDI Kit HisCube - Ni-INDIGO His-Tag Protein...
Fast & easy | Our HisCube MIDI Kit for MIDI sized His-tag protein purifications assays. | For maximum protein purity & yield
Article number: 80105
Sales price: €454.00 *
PureCube Ni-IDA Agarose PureCube Ni-IDA Agarose
Ni-IDA Agarose resin for His-tag protein purification. Ø 40 µm bead diameter. Great protein yield. For batch spin & FPLC!
Article number: 30103
Sales price: From €73.00 *
PureCube Compact Cartridge Co-NTA PureCube Compact Cartridge Co-NTA
COMPACT Cartridges / Columns for FPLC based His-tag Protein Purification. Pre-Filled with Co-NTA Agarose (40µm).
Article number: 31602
Sales price: From €39.00 *
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