Strep-Tactin® 4Flow® high capacity FPLC column
Order number: 2-1257-001
| Quantity | Unit price |
|---|---|
| 1 - 9 |
€141.00*
|
| From 10 |
€133.00*
|
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Description
Please note: this product replaces PureCube HiCap Strep-Tactin® FPLC column (Cat. No. 34302 & 34306).
Strep-Tactin® 4Flow® high capacity FPLC column is identical to PureCube HiCap Strep-Tactin® FPLC column.
Strep-Tactin® 4Flow® high capacity FPLC columns are ready-to-use columns for affinity
chromatography. They are suitable for automated protein purification with HPLC/FPLC devices such as
Äkta® systems (Cytiva). The FPLC columns contain a cross-linked agarose coupled to the ligand Strep -Tactin®, a streptavidin variant that binds to Strep-tag®II or Twin-Strep-tag® fusion proteins.
This protein purification system is characterized by a high target purity of >95% and a stable
performance even after several regeneration cycles.
These columns are the perfect alternative for proteins that are difficult to elute from Strep-Tactin®XT 4Flow® and Strep-Tactin®XT 4Flow® high capacity FPLC columns.
Datasheets
| Feature - Strep-Tactin® XT 4Flow® high capacity FPLC column | |
|---|---|
| Usage | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity | Twin-Strep-tag® and Strep-tag®II |
| Binding capacity | 31 mg/ml |
| Bead Ligand | Strep-Tactin®XT |
| Bead size | 50-150 µm |
| Matrix | 4% agarose, crosslinked |
| Filling quantity | 1 ml pre-packed gravity flow column |
| Storage Conditions | 2-8°C |
| Shipping Conditions | RT |
Citations
FAQ
Can I get the datasheet for the Strep-Tactin®XT 4Flow® high capacity FPLC column?
Yes. Just click here to download it as a PDF File.
Is Strep-Tactin® the same as Streptavidin?
No, they are not. Strep-Tactin® is a bioengineered protein, originally based on Streptavidin. However, Strep-Tactin® cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.
Disclaimer
Strep-Tactin® and Strep-tag® are trademarks of IBA GmbH. PureCube Strep-Tactin® Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".