PureCube NTA Agarose XL
Order number: 55810
Description
PureCube NTA XL (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 400 µm. This is very large and is meant for very high flow rates in e.g. FPLC assays or very viscous cell media.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.
PureCube NTA resin is also available with a bead diameter of 40 µm or 100 µm for greater mechanical stability or as NTA magnetic beads.
However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.
PureCube NTA resin is also available with a bead diameter of 40 µm or 100 µm for greater mechanical stability or as NTA magnetic beads.
However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
- His-tag purification: Ni-NTA, Co-NTA, Zn-NTA, and Cu-NTA
- Phosphopeptide Enrichment: Al-NTA, Fe-NTA, Ti-NTA, and Zr-NTA. (Ti and Zr only as MagBeads)
| Feature | |
|---|---|
| Usage | For coupling of metal ions for corresponding purification and / or enrichment purposes |
| Specificity | Varies |
| Binding capacity | Depends on the loaded ion |
| Purity | Depends on the loaded ion |
| Bead Ligand | NTA |
| Bead size | 400 μm |
| Matrix | 7.5% cross-linked agarose |
| Filling quantity | Delivered as a 50 % suspension |
| Recommended FPLC flow rates | 0.5-2 ml/min |
| Maximal FPLC flow rate | 6 ml/min |
| pH stability | 2-14 |
| Other stabilities | 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile |
FAQ
Can I get the datasheet for the PureCube NTA XL resin?
Why is there a special protocol for the loading of nickel ions?
As Ni-NTA is the most commonly used NTA product, we have optimized this specific protocol.
Can I order a specific NTA product from you, even if it is not in your shop?
Yes, we offer a custom bead service for that.