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Bacteriorhodopsin (Hs)

Order number: 28903
Bacteriorhodopsin (Hs)

€515.00*

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Description

Bacteriorhodopsin (BR) is a 7-transmembrane protein that acts as a light-driven proton pump in archaebacteria. Due to its covalently bound colored ligand, BR is widely used as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide BR of high purity, prepared as purple membrane patches from Halobacterium salinarum.

Datasheets

Feature
Alternative names Bacteriorhodopsin, BR, HsBR, Bacteriopsin
UniProt Number P02945
Protein class 7-transmembrane protein
Organism Halobacterium salinarum S9
Sequence, explained Full-length, wildtype sequence with N-terminal signal sequence which is mostly absent in the preparation
Sequence, One-Letter Code MLELLPTAVE GVSQAQITGR PEWIWLALGT ALMGLGTLYF LVKGMGVSDP DAKKFYAITT LVPAIAFTMY LSMLLGYGLT MVPFGGEQNP IYWARYADWL FTTPLLLLDL ALLVDADQGT ILALVGADGI MIGTGLVGAL TKVYSYRFVW WAISTAAMLY ILYVLFFGFT SKAESMRPEV ASTFKVLRNV TVVLWSAYPV VWLIGSEGAG IVPLNIETLL FMVLDVSAKV GFGLILLRSR AIFGEAEAPE PSAGDGAAAT SD
Affinity tags None
Expression Host Halobacterium salinarum S9
Size (excluding additional elements) 262 amino acids; 28,256 Da
Buffer composition Purple membrane (PM) patches in H2O, 0.02% NaN3
Purity >95%, determined via SDS-PAGE
Purified via Sequential centrifugation
Absorbance Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Activity Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 560 nm = 2.3
Function Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven proton pump
Literature references
  1. Kubicek, Jan, et al. "Controlled in meso phase crystallization–a method for the structural investigation of membrane proteins." PloS one 7.4 (2012): e35458.
  2. Zscherp, Christian, Ramona Schlesinger, and Joachim Heberle. "Time-resolved FT-IR spectroscopic investigation of the pH-dependent proton transfer reactions in the E194Q mutant of bacteriorhodopsin." Biochemical and biophysical research communications 283.1 (2001): 57-63.

Citations

Lab Results

BR westernblot
Figure 1: SDS-PAGE of bacterio-rhodopsin in purple membrane patches from H. salinarum.
BR absoprtion spectrum measured by Cube Biotech
Figure 2: Absorbance spectrum of bacteriorhodopsin in purple membrane patches from H. salinarum.

FAQ

Can I get the datasheet for the Bacteriorhodopsin protein?

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Yes, we do. Just browse through our product protofolio. In case we do not have your desired membrane protein in our store, send a request to our protein services.