Sulfo-SMA

Order number: 18521

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Description

Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another major advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:

Datasheets

Features
Usage	Membrane protein solubilization & stabilization
Full-Name	Poly(styrene-co-N′,N′-dimethyl(maleimidoethyl)ammonium propane sulfonate) copolymer
Formula Weight	8,000 g/mol ± 1000 g/mol
Buffer	50 mM HEPES
pH upon addition of dH₂O	7.5 ± 0.3
Total pH stability	4-10
State	Lyophilized Powder
Recommended concentration range upon use	1% to 5%
Solubility	> 10 % in H₂O
Mg²⁺ Tolerance	over 100 mM
Ca²⁺ Tolerance	over 100 mM
Color	White to slightly yellow
Odor	Odorless
Shipping Temperature	ambient temperature
Long-term Storage (lyophilized copolymer)	-20°C for several years
Short-term Storage (dissolved copolymer)	2-8°C for several days

Lab Results

Sulfo-SMALP chemical stucture — Fig. 1: The chemical structure of a Sulfo-SMALP monomere.

Sulfo Dibma Westernblot — Fig. 2: Shown is the blot activity of a Rho-Tag purified human membrane protein expressed in HEK cells containing 9 transmembrane helices. The membrane protein was solubilized using Sulfo-SMA as well as other polymers from the SMA family. The electroneutral Sulfo-SMA exhibits the highest solubilization and purification efficiency of all testes SMAs. The purified membrane protein is stabilized in an electroneutral polymer belt.

Sulfo Polymers Net Charge — Fig. 3: ζ-potential of DMPC nanodiscs formed with different polymers. Ultrasolute 18, DIBMA 10 and SMA 200 nanodiscs reveal a strong negative net charge whereas nanodiscs formed with Sulfo DIBMA and Sulfo SMA reveal a nearly electroneutral nanodisc compared to non-sulfonated controls. ζ-potential was measured in 150 mM NaCl, 20 mM HEPES at pH 7.5 and a Polymer/DMPC weigth ratio of 3/1.

Comparison of Hdrophobicity — Fig. 4: Hydrodynamic size of DMPC nanodiscs formed with different polymers. Nanodisc size was measured in 150 mM NaCl, 20 mM HEPES at pH 7.5 and a Polymer/DMPC weigth ratio of 3/1.

Sulfo Polymers CA 2+ Tolerance — Fig. 5: Ca²⁺ tolerance of Sulfo-Polymers. No polymer precipitation occurs up to 100 mM Ca²⁺ .

Sulfo Polymers MG 2+ Tolerance — Fig. 6: Mg²⁺ tolerance of Sulfo-Polymers. No polymer precipitation occurs up to 100 mM Mg²⁺.

Video

Watch our video on the use of synthetic polymers for membrane protein solubilization. It demonstrates the use with SMALP.

FAQ

Can I have the datasheet for Sulfo-SMA?

Yes of course, you can download it here.

Is Sulfo-SMA from Cube Biotech ready to use?

Yes, our Sulfo-SMA is ready to use. You can start directly with the solubilization. Read our protocol for more information.

Which pH is suitable for Sulfo-SMA ?

For Sulfo-SMA a pH of 7.5 is recommended.

Which concentrations of Sulfo-SMA should I use for my protein?

In general, we advise you to add 2,5 % Sulfo-SMA to your solution. But the optimal conditions have to be screened by yourself. In general you should not go below 1% or over 5%. Please refer to our Membrane Protein Solubilization Protocol for more information. Or simply ask our support team.

Disclaimer

Patent Pending
The product described above is based on a substance published within the work of D. Glueck et al., Small, 2022, 2202492, DOI: 10.1002/smll.202202492.
Our products are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.