Protein Purification Products


Purification Resins
Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer. 

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His-tagged proteins
Glutathione / GST-tag Agarose & MagBeads The GST-tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST-tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific. Strep-tagged proteins
Rho1D4 / 1D4-tag Affinity Agarose & MagBeads The-Rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! Rho1d4-tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
Protein Purification Products
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PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Ni-NTA Agarose resin for His-tag protein purification. Ø 100 µm bead diameter. Great protein yield. For batch spin & FPLC!
Article number: 74103
Sales price: From €112.00 *
Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
For the detection of Rho1D4 tagged proteins on westernblots. Our Rho1D4 affinity antibody, from mouse!
Article number: 40020
Sales price: €288.00 *
Strep antibody (0.1 mg) Strep antibody (0.1 mg)
For the detection of Strep tagged proteins on westernblots. Our Strep affinity antibody, from mouse!
Article number: 40070
Sales price: €202.00 *
PureCube Carboxy MagBeads PureCube Carboxy MagBeads
Protein purification ► Carboxygroup-carrying Magnetic Beads, for activation by NHS or weak ion-exchaning ★25% conc.★
Article number: 50201
Sales price: From €88.00 *
PureCube NHS Activated Agarose PureCube NHS Activated Agarose
Ø 40µm high-quality NHS activated agarose resin beads for the coupling of biomolecules via exposed amine (NH2) groups.
Article number: 50303
Sales price: From €206.00 *
PureCube Maleimide Activated Agarose PureCube Maleimide Activated Agarose
Ø 40µm high-quality Maleimide activated agarose resin beads for the coupling of biomolecules via exposed SH (Thiol) groups.
Article number: 51103
Sales price: From €311.00 *
Rho1D4 peptide Rho1D4 peptide
Ultra-Pure Rho1D4 peptide. | For Rho1D4-tag protein elution from Rho1D4 agarose or MagBeads | Ready for your research.
Article number: 16201
Sales price: From €111.00 *
Cube MagBead Separator (1) Cube MagBead Separator (1)
1 Cube MagBead Separator, for 2 microtubes of 1.5 or 2 ml volume.
Article number: 16941
Sales price: From €65.00 *
PureCube NTA MagBeads PureCube NTA MagBeads
NTA magnetic beads for your customized protein purification or enrichment. Create your desired magnetic beads.
Article number: 31801
Sales price: From €65.00 *
PureCube Glutathione MagBeads PureCube Glutathione MagBeads
Cube Biotech offers PureCube GST MagBeads for the protein purification of GST-tagged proteins | 10 mg protein / ml MagBeads
Article number: 32205
Sales price: From €52.00 *
PureCube Rho1D4 Agarose PureCube Rho1D4 Agarose
Our PureCube Rho1D4 agarose for protein purification. Rho1D4antibody agarose beads for maximum purity and specificity.
Article number: 33101
Sales price: From €239.00 *
PureCube Rho1D4 MagBeads PureCube Rho1D4 MagBeads
PureCube magnetic beads with Rho1D4 / 1D4-tag affinity. For highly specific and ~100% pure membrane protein purifications.
Article number: 33201
Sales price: From €254.00 *
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