Protein Purification Products


Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used in together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer.

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His tagged proteins
Glutathione / GST tag Agarose & MagBeads The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific./td> Strep tagged proteins
Rho1D4 / 1D4 tag Affinity Agarose & MagBeads The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! rho1d4 tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS Activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine Activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy Activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide Activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
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Protein Purification Products
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PureCube Glutathione MagBeads PureCube Glutathione MagBeads
1 ml 25% Glutathione Magnetic Beads For more information read here: https://cube-biotech.com/products/magnetic-beads/gst-affinity-magbeads/#
Article number: 32205
Sales price: From €50.00 *
PureCube HiCap StrepTactin MagBeads PureCube HiCap StrepTactin MagBeads
StrepTactin ® Magnetic Beads for purification of proteins through the Strep ® tag. High affinity and protein yield.
Article number: 34201
Sales price: From €78.00 *
Rho1D4 peptide Rho1D4 peptide
Ultra-Pure Rho1D4 peptide. | For Rho1D4 tag protein elution from Rho1D4 agarose or MagBeads | Ready for your research.
Article number: 16201
Sales price: From €107.00 *
PureCube Co-NTA MagBeads PureCube Co-NTA MagBeads
1 ml 25% NTA magnetic beads, loaded with cobalt (II) chloride
Article number: 31501
Sales price: From €68.00 *
Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
0.2 mg Rho1D4 antibody in solution  
Article number: 40020
Sales price: €279.00 *
PureCube NHS Activated MagBeads PureCube NHS Activated MagBeads
Protein purification ► NHS-Activated Magnetic Beads, for direct coupling of biomolecules via amine groups. ★25% conc.★
Article number: 50401
Sales price: From €110.00 *
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
2 ml 50% NTA Agarose, with 100 micron average bead size. Loaded with nickel (II) sulfate
Article number: 74103
Sales price: From €108.00 *
PureCube Fe-NTA Agarose PureCube Fe-NTA Agarose
Our PurceCube NTA-Agarose loaded with iron (III) chloride. Perfect for purification of phosphorylated proteins. 20ml
Article number: 31403-Fe
Sales price: From €107.00 *
PureCube Al-NTA MagBeads PureCube Al-NTA MagBeads
1 ml 25% NTA magnetic beads, loaded with aluminium (III) chloride
Article number: 31501-Al
Sales price: From €68.00 *
PureCube Zn-NTA Cartridge PureCube Zn-NTA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with zinc (II) chloride
Article number: 31601-Zn
Sales price: From €37.00 *
PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
25% concentrated INDIGO-Ni agarose for his tag protein purification. EDTA and DTT stable. ~100% purity for your his tagged protein.
Article number: 75201
Sales price: From €69.00 *
PureCube Ni-IDA Agarose PureCube Ni-IDA Agarose
2 ml 50% IDA Agarose, loaded with nickel (II) sulfate
Article number: 30103
Sales price: From €70.00 *
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