Protein Purification Products


Purification Resins
Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer. 

Not sure which tag to use? See our affinity tag overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His-tagged proteins
Glutathione / GST-tag Agarose & MagBeads The GST-tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST-tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific. Strep-tagged proteins
Rho1D4 / 1D4-tag Affinity Agarose & MagBeads The-Rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! Rho1d4-tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
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Protein Purification Products
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Rho Starter Set 4: PureCube Compact Cartridge Rho1D4 (1x1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 4: PureCube Compact Cartridge...
Cube Biotech offers Rho1D4-tag affinity COMPACT columns & the Rho1D4 protein elution peptide together as an affordable set.
Article number: 33398
Sales price: €205.00 * €410.00 *
PureCube HiCap StrepTactin MagBeads PureCube HiCap StrepTactin MagBeads
StrepTactin ® Magnetic Beads for purification of proteins through the Strep®-tag. High affinity and protein yield.
Article number: 34201
Sales price: From €81.00 *
L-Glutathione reduced L-Glutathione reduced
Reduced L-Glutathione ►Ultrapure and convenient for GST-tagged protein elution from Glutathione Agarose or MagBeads.
Article number: 61035
Sales price: €115.00 *
PureCube Fe-NTA Agarose PureCube Fe-NTA Agarose
PureCube High quality Fe-NTA agarose resin for the enrichment of phosphorylated peptides. | Automated assays possible
Article number: 31403-Fe
Sales price: From €111.00 *
Rho Starter Set 2: PureCube Rho1D4 MagBeads (1 ml) + Rho1D4 peptide (5 mg) Rho Starter Set 2: PureCube Rho1D4 MagBeads (1...
Cube Biotech offers Rho1D4-tag affinity MagBeads & the Rho1D4 protein elution peptide together as an affordable set.
Article number: 33299
Sales price: €182.00 * €364.00 *
Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
For the detection of Rho1D4 tagged proteins on westernblots. Our Rho1D4 affinity antibody, from mouse!
Article number: 40020
Sales price: €288.00 *
Cube MagBead Separator (1) Cube MagBead Separator (1)
Our Separator for magnetic beads comes with a practical ★modular★ design and strong magnets. No loss of magnetic bead again.
Article number: 16941
Sales price: From €65.00 *
PureCube Co-NTA Agarose PureCube Co-NTA Agarose
Co-NTA Agarose resin for His-tag protein purification. Ø 40 µm bead diameter. Great protein purity. For batch spin & FPLC!
Article number: 31403
Sales price: From €111.00 *
PureCube NHS Activated Agarose PureCube NHS Activated Agarose
Ø 40µm high-quality NHS activated agarose resin beads for the coupling of biomolecules via exposed amine (NH2) groups.
Article number: 50303
Sales price: From €206.00 *
PureCube NHS Activated MagBeads PureCube NHS Activated MagBeads
Protein purification ► NHS-Activated Magnetic Beads, for direct coupling of biomolecules via amine groups. ★25% conc.★
Article number: 50401
Sales price: From €206.00 *
PureCube 100 INDIGO Ni-Cartridge PureCube 100 INDIGO Ni-Cartridge
Our exclusive & high quality INDIGO-Ni agarose resin pre-filled in FPLC Cartridges / Columns for your His Tag purifications!
Article number: 75301
Sales price: From €50.00 *
PureCube Ni-NTA Cartridge PureCube Ni-NTA Cartridge
Cube Biotech offers Cartridges / Columns for FPLC based His-tag Protein Purification. Pre-Filled with Ni-NTA Agarose (40µm).
Article number: 31301
Sales price: From €36.00 *
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