Protein Purification Products


Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used in together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer.

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His-tagged proteins
Glutathione / GST tag Agarose & MagBeads The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST-tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific. Strep-tagged proteins
Rho1D4 / 1D4 tag Affinity Agarose & MagBeads The-Rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! rho1d4 tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
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Protein Purification Products
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Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
For the detection of Rho1D4 tagged proteins on westernblots. Our Rho1D4 affinity antibody, from mouse!
Article number: 40020
Sales price: €279.00 *
PureCube NHS Activated Agarose PureCube NHS Activated Agarose
Ø 40µm high-quality NHS activated agarose resin beads for the coupling of biomolecules via exposed amine (NH2) groups.
Article number: 50303
Sales price: From €117.00 *
PureCube Cu-NTA MagBeads PureCube Cu-NTA MagBeads
For Copper-Binding and for His-tagged protein. | Cu-NTA agarose for highest protein yield of his tagged proteins.
Article number: 31501-Cu
Sales price: From €68.00 *
Cube MagBead Separator (1) Cube MagBead Separator (1)
1 Cube MagBead Separator, for 2 microtubes of 1.5 or 2 ml volume.
Article number: 16941
Sales price: From €63.00 *
PureCube IDA MagBeads PureCube IDA MagBeads
IDA agarose magnetic beads for your customized protein purification or enrichment. Create your desired magnetic beads.
Article number: 30801
Sales price: From €43.00 *
PureCube HiCap StrepTactin Agarose PureCube HiCap StrepTactin Agarose
High-quality StrepTactin® Agarose Resin beads for the purifcation of Strep® tagged proteins. | For FPLC and Batch Spin
Article number: 34101
Sales price: From €70.00 *
PureCube Maleimide Activated MagBeads PureCube Maleimide Activated MagBeads
Protein purification ► Maleimide-Activated Magnetic Beads, for direct coupling of biomolecules via thiol groups. ★25% conc.★
Article number: 51201
Sales price: From €138.00 *
PureCube Fe-NTA Agarose PureCube Fe-NTA Agarose
PureCube High quality Fe-NTA agarose resin for the enrichment of phosphorylated peptides. | Automated assays possible
Article number: 31403-Fe
Sales price: From €107.00 *
PureCube Ti-NTA MagBeads PureCube Ti-NTA MagBeads
PureCube Ti-NTA magnetic beads for the enrichment of phoposphopeptides. Compatible with automated assays.
Article number: 31501-Ti
Sales price: From €68.00 *
PureCube Zr-NTA MagBeads PureCube Zr-NTA MagBeads
PureCube Zr-NTA magnetic beads for the enrichment of phoposphopeptides. Compatible with automated assays.
Article number: 31501-Zr
Sales price: From €68.00 *
PureCube Ni-NTA Cartridge PureCube Ni-NTA Cartridge
Cube BIotech offers Cartridges / Columns for FPLC based His tag Protein Purification. Pre-Filled with Ni-NTA Agarose (40µm).
Article number: 31301
Sales price: From €34.00 *
PureCube Glutathione Agarose PureCube Glutathione Agarose
Cube Biotech offers PureCube GST Agarose resin for the protein Purification of GST tagged proteins | 10 mg protein / ml resin
Article number: 32103
Sales price: From €112.00 * €140.00 *
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