PureCube Amine Activated Agarose
Order number: 51003
Description
PureCube Amine Activated Agarose resin is your best option to couple biomolecules that carry free-standing carboxy (COOH) groups. The resulting peptide bond between the Amine Agarose beads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube Amine Activated Agarose is also available as magnetic beads in case you prefer this purification method, or as XL agarose resin if you need higher flow rates for your downstream application.
| Feature | |
|---|---|
| Usage | Coupling proteins to active Amine Groups |
| Specificity | Exposed carboxy (COOH) groups on biomolecule |
| Amine groupo density | higher than 20 µmol/ml |
| Bead Ligand | Chemically active Amine groups |
| Bead size | 40 μm |
| Filling quantity | Delivered as a 50 % suspension |
| Storage Buffer | 100% isopropanol |
| Matrix | 7.5% cross-linked agarose |
| Short Term storage | In neutral buffer at 4°C |
| Long Term storage | 100 mM sodium hydrogen carbonate, 0.02% sodium azide, pH 7.5 at 4 °C or 20 mM sodium acetate, 20% ethanol, pH 6.5 at 4 °C |
| Shelf life | If properly stored - 3 years upon delivery, unopened |
| Chemical Structure |  |
Citations
| Coupled biomolecule | Year | Author |
|---|---|---|
| l-lactate oxidase | 2024 | Luley-Goedl C., Bruni M., Nidetzky B. |
FAQ
Can I get the datasheet for the Amine Activated Agarose?
What can I do with these beads?
The beads carry reactive Amine groups on their surface. These can be used to bind all sorts of biomolecules if they carry free Carboxy (COOH) groups. The subsequent use of the beads depends on what biomolecule was coupled.
How does the coupling work?
How long can I store the Amine Activated Beads?
Unlike some other activated beads, amine activated beads have a long shelf life. The half-life of the amine beads is around 3 years of properly stored.