PureCube Maleimide Activated MagBeads
Order number: 51225
Description
PureCube Maleimide MagBeads are your best option to couple biomolecules that carry thiol (SH) groups. The resulting Thioether bond between the Maleimide Magbeads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube Maleimide Activated MagBeads are also available as agarose resin in case you prefer this method catalog, or as XL MagBeads if you need higher flow rates for your downstream application.
| Feature | |
|---|---|
| Usage | Coupling proteins to active Maleimide Groups |
| Specificity | Exposed thiol (SH) groups on biomolecule |
| Bead Ligand | Chemically active Maleimide groups |
| Resulting Bond | irreversible Thioether |
| Protein binding capacity | ≥15 mg/ml |
| Bead size | 30 μm |
| Filling quantity | Delivered as a 25 % suspension |
| Matrix | 6% cross-linked agarose |
| Short Term Storage | In neutral buffer at 4°C |
| Long Term Storage | 2-propanol, anhydrous |
| Shelf life | If properly stored - 3 months upon delivery, unopened |
| Chemical Structure |  |
Citations
| Coupled biomolecule | Year | Author |
|---|---|---|
| Aptamer Candidates | 2021 | Shkembi X., Skouridou V., Svobodova M., Leonardo S., Bashammakh A.S., Alyoubi A.O., Campàs M., O'Sullivan C.K. |
| sSrc conjugation | 2023 | Chang A., Leutert M., Rodriguez-M R.A., Villén J. |
| Oligonucleotides | 2023 | Campàs M., Leonardo S., Ferré-Gode A., Chowdhury A.A. Toldrà A., Andree K.B., Roque A. |
FAQ
Can I get the datasheet for the Maleimide Activated MagBeads?
What can I do with these beads?
The beads carry reactive Maleimide groups on their surface. These can be used to bind all sorts of biomolecules if they carry free thiol (SH) groups. The subsequent use of the beads depends on what biomolecule was coupled. Note: The maleimide coupling is irreversible! If you need reversible thiol coupling beads, we recommend our Epoxy Activated beads.
How does the coupling work?
How long can I store the Epoxy Agarose?
The reactive properties of the Maleimide beads are very potent. But therefore, they degrade over time. We can safely say that upon delivery the beads last for up to 3 months. If properly stored of course.