Our Maleimide Activated Agarose resins and MagBeads are carrying an Maleimide functionality. With this activated maleimide groups can be bound to the beads covalently via thiol groups. Therefore the subsequent use of our Maleimide Beads is dependend on your coupled biomolecule.
Fig. 1: Structural formula of a maleimide group
Overview of Cube Biotech's Maleimide Activated Beads for biomolecule coupling via carboxy groups.
Features
| Usage | Coupling proteins to via their activated thiol groups |
| Specifity | Affinity to thiol groups |
| Bead Ligand | Affinity agarose with activated Maleimide groups |
Maleimide acivated agarose resins and MagBeads from Cube Biotech were successfully used in the following publications and patents:
| Targeted Protein | Activated chemical group | Year | Author | Product |
|---|
| cap-specific nucleoside 2′-O-methyltransferase. | Maleimide | 2016 | Roos T., Yazdan Oanah B., Conzelmann M., Thess A., Buob D., Kunze M., Wagner V.2016-August-121 | Maleimide Agarose resin [40µm] |
| Recombinant mutant PPases | Maleimide | 2018 | Kunze M., Hadler F.N., Yazdan Panah B.,Ross T. 2 | Maleimide Agarose resin [40µm] |
| P2-GM1os | Maleimide | 2019 | Mahon C.S., Wildsmith G.C., Haksar D., de Poel E., Beekman J.M., Pieters R.J., Webb M.E., Turnbill W.B.3 | Maleimide Agarose resin [40µm] |
| Aptamer Candidates | Maleimide | 2021 | Shkembi X., Skouridou V., Svobodova M., Leonardo S., Bashammakh A.S., Alyoubi A.O., Campàs M., O'Sullivan C.K..4 | Maleimide MagBeads [30µm] |
References
- Roos T., Yazdan Oanah B., Conzelmann M., Thess A., Buob D., Kunze M., Wagner V. inventor; Method for adding cap structures to rna using immobilized enzymes. International patent: WO2016193226A1. 2016-December-08
- Kunze M., Hadler F.N., Yazdan Panah B.,Ross T. inventor; CureVae AG, Tübingen, Immobilized Inorganic Pyrophosphatase (Ppase), Us-Patent: US 2019 / 0177714 A1; September-21-2018
- Mahon S. et al. (2019). A ‘catch-and-release’ receptor for the cholera toxin. Faraday Discussions. 10.1039/C9FD00017H.
- Xhensila et al. (2021) Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish Analytical Chemistry Article ASAP DOI: 10.1021/acs.analchem.1c03671
