Maleimide Activated


Purification Resins
Our Epoxy Activated Agarose resind and MagBeads are carrying an epoxy functionality. With this activated epoxy group Proteins can be bound to the beads covalently via free amines, thiol groups or hydroxyl groups. Therefore the subsequent use of our Epoxy Beads is dependend on your coupled biomolecule.

Overview of Cube Biotech's Maleimide Activated Beads for biomolecule coupling via carboxy groups.

Amine Acfticated AgaroseAmine Activated MagBeads
Maleimide agarose resin (40 µm) Maleimide Activated MagBeads (30 µm)
Maleimide Activated Agarose Resin (XL sized) Maleimide Activated MagBeads (XL sized)

Features

Usage Coupling proteins to via their activated thiol groups
Specifity Affinity to thiol groups
Bead Ligand Affinity agarose with activated Maleimide groups

Maleimide acivated agarose resins and MagBeads from Cube Biotech were successfully used in the following publications and patents:

Targeted ProteinActivated chemical groupYearAuthorProduct
cap-specific nucleoside 2′-O-methyltransferase. Maleimide 2016 Roos T., Yazdan Oanah B., Conzelmann M., Thess A., Buob D., Kunze M., Wagner V.2016-August-121 Maleimide Agarose resin [40µm]
Recombinant mutant PPases Maleimide 2018 Kunze M., Hadler F.N., Yazdan Panah B.,Ross T. 2 Maleimide Agarose resin [40µm]
P2-GM1os Maleimide 2019 Mahon C.S., Wildsmith G.C., Haksar D., de Poel E., Beekman J.M., Pieters R.J., Webb M.E., Turnbill W.B.3 Maleimide Agarose resin [40µm]

References

  1. Roos T., Yazdan Oanah B., Conzelmann M., Thess A., Buob D., Kunze M., Wagner V. inventor; Method for adding cap structures to rna using immobilized enzymes. International patent: WO2016193226A1. 2016-December-08
  2. Kunze M., Hadler F.N., Yazdan Panah B.,Ross T. inventor; CureVae AG, Tübingen, Immobilized Inorganic Pyrophosphatase (Ppase), Us-Patent: US 2019 / 0177714 A1; September-21-2018
  3. Mahon S. et al. (2019). A ‘catch-and-release’ receptor for the cholera toxin. Faraday Discussions. 10.1039/C9FD00017H.
Maleimide Activated
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PureCube Maleimide Activated Agarose PureCube Maleimide Activated Agarose
2 ml 50% Maleimide-Activated Agarose, for direct, irreversible coupling of biomolecules via thiol groups For more information read here:...
Article number: 51103
Sales price: From €301.00 *
PureCube Maleimide Activated MagBeads PureCube Maleimide Activated MagBeads
1 ml 25% Maleimide-Activated MagBeads, for direct, irreversible coupling of biomolecules via thiol groups  
Article number: 51201
Sales price: From €138.00 *
PureCube Maleimide Activated Agarose XL PureCube Maleimide Activated Agarose XL
20 ml 50% Maleimide-Activated Agarose, for direct, irreversible coupling of biomolecules via thiol groups. Medium particle size of 400 micrometer, for special applications
Article number: 59103
Sales price: From €395.00 *
PureCube Maleimide Activated MagBeads XL PureCube Maleimide Activated MagBeads XL
1 ml 25% Maleimide-Activated MagBeads, for direct, irreversible coupling of biomolecules via thiol groups.Particle size of 70-120 micrometer, for special applications
Article number: 59205
Sales price: From €710.00 *
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