NativeMP™ Strep-Tactin®XT Purification Plate , 96 deep-well plate (KingFisher™)

Order number: 90422

Quantity	Unit price
1 - 4	€1,386.00*
5 - 9	€1,247.00*
From 10	€1,178.00*

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Description

NativeMP™ Strep-Tactin®XT Purification plates are 96 deep-well plates for high-throughput membrane protein purification of Strep-tag®II or Twin-Strep-tag® fusion proteins with KingFisher instruments. They contain MagStrep® Strep-Tactin®XT beads, which are characterized by a high binding specificity and affinity. The plates have to be combined with NativeMP™ Screening plates (Cat. No. 90302, 90304 or 90306) to identify optimal solubilization and stabilization conditions for a membrane protein of choice.

Datasheets

Content and setup of a NativeMP™ Purification Plate, 96 deep-well plate:

	Content:
	MagStrep® Strep-Tactin®XT beads

Overview of NativeMP™ Strep-Tactin®XT Purification Plate contents — Figure 1: NativeMP™ Strep-Tactin®XT Purification Plate

Other Features
Plate type	KingFisher™ 96 deep-well plate (Cat. No. 95040450)
Bead size	20-40 µm (30 µm average)
Bead volume	30 µL dehydrated magnetic beads per well
Binding capacity	Up to 1.3 mg protein per well. Binding capacity was determined with a 50 kDa Twin-Strep-tag® fusion protein. Please note that the binding capacity is highly protein-dependent.
Working volume	50-950 μl per well
Working pH	Optimal: pH 7-8
Application	High-throughput membrane protein purification in 96 well format. For membrane protein solubilization & stabilization and subsequent purification, use NativeMP™ Screening Plate in combination with NativeMP™ Strep-Tactin®XT Purification Plate. Compatible with KingFisher Flex (program & protocol provided), Duo Prime and Presto instruments.
Stability	6 months after shipping
Storage	2-8 °C
Shipping	Room temperature
Hazards	Product is not classified as hazardous according to (EC) No 1272/2008 [CLP]. A Material Safety Data Sheet is provided.
Manufacturer	Cube Biotech

Citations

Lab Results

Figure 2:
Quantitative analysis of membrane protein content and quality by SDS–PAGE, 330nm fluorescence and DLS. a.+ d. Solubilization and purification efficiency was screened for 14 MPs with 15 different NativeMPTM copolymers. Eluates were run an SDS–PAGEs (Coomassie stained). c. + e. Protein quantity was analyzed either by densitometry (ImageJ Gel Analyzer) or by buried tryptophan fluorescence at 330 nm (NanoTemper Panta Discovery). Intensities were normalized to the strongest signal in each dataset (set to 100%) and plotted as box plots to visualize distributions across copolymer families. d. For HTR7, eluates from all tested copolymers are shown (a), while for the remaining targets only the strongest eluates are displayed. Full, unmodified SDS– PAGEs and Western blots are provided in Fig. S1-3. In panel e, results from all 14 targets were combined to compare solubilization and stabilization efficiencies across the six copolymer backbones. Panel b shows DLS of HTR7 eluates, with hydrodynamic radius (x-axis) plotted against PDI (y-axis). (Source: Hanisch et al., 2025 https://doi.org/10.1101/2025.09.05.674548)

Disclaimer

Trademark information

The owners of trademarks marked by “®” or “TM” are identified at https://www.cube-biotech.com/information/patents. Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law.

Trademark	Current Owner
KINGFISHER	Thermo Fisher Scientific Inc.

Disclaimer: KingFisher is a trademark of Thermo Fisher Scientific Inc. Cube Biotech is not affiliated with Thermo Fisher Scientific Inc.

Important licensing information

This product is covered by intellectual property (IP) rights and on completion of the sale Cube Biotech grants respective Limited Use Label Licenses to purchaser. IP rights and Limited Use Label Licenses for said technology are further described and identified at https://www.cube-biotech.com/information/patents or upon inquiry at contact@cube-biotech.com or at Cube Biotech GmbH, Creative-Campus-Allee 12, D-40789 Monheim, Germany. By use of this product the purchaser accepts the terms and conditions of all applicable Limited Use Label Licenses.