Strep-Tactin®XT 4Flow® high capacity column
Order number: 2-5032-001
Description
The Strep-Tactin®XT 4Flow® high capacity gravity flow columns can be used for the purification of proteins that have been tagged with Strep-tag®II or Twin-Strep-tag®. These fusion proteins bind very specifically to the high affinity Strep-Tactin®XT ligand resulting in highly pure target proteins (>95%). It is recommended for challenging and low abundant proteins because of its stability and low agarose concentration. For proteins with increased affinity, like multimeric proteins, Strep-Tactin® resins for easier elution are recommended.
Datasheets
| Feature - Strep-Tactin® XT 4Flow® high capacity column | |
|---|---|
| Usage | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity | Twin-Strep-tag® and Strep-tag®II |
| Binding capacity | >31 mg/ml |
| Bead Ligand | Strep-Tactin® |
| Bead size | 50-150 µm |
| Matrix | 4% agarose, crosslinked |
| Filling quantity | 1 ml pre-packed gavity flow column |
| for Storage at Cube Inc. | 4°C |
| for storage at Cube GmbH (Monheim) | 4°C |
| Storage Conditions | 2-8°C |
| Shipping Conditions | RT |
FAQ
Can I get the datasheet for the Strep-Tactin®XT 4Flow® high capacity column?
Yes. Just click here to download it as a PDF File.
Is Strep-Tactin® the same as Streptavidin?
No, they are not. Strep-Tactin® is a bioengineered protein, originally based on Streptavidin. However, Strep-Tactin® cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.
Disclaimer
Strep-Tactin® and Strep-tag® are trademarks of IBA GmbH. PureCube Strep-Tactin® Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".