Strep-Tactin®XT 4Flow® high capacity FPLC column
Order number: 2-5028-001
Description
Strep-Tactin®XT 4Flow® high capacity FPLC columns contain 4% agarose resin, coupled to Strep‑Tactin®XT. The columns can be used for fully automated purification of Strep-tag®II or Twin-Strep-tag® fusion proteins with HPLC/FPLC devices, like Äkta®. The resin is suitable for all protein classes, especially low abundant or challenging proteins. The low concentration of agarose is suitable for the purification of targets with high molecular weights. In comparison to Strep-Tactin® 4Flow® resin, this high capacity variant has higher density of Strep-Tactin®XT, resulting in twice as much purified protein. The resin has broad pH compatibility, excellent pressure stability, strong binding affinity and delivers high target purity (> 95 %).
Datasheets
| Feature - Strep-Tactin® XT 4Flow® high capacity FPLC column | |
|---|---|
| Usage | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity | Twin-Strep-tag® and Strep-tag®II |
| Binding capacity | 31 mg/ml |
| Bead Ligand | Strep-Tactin® |
| Bead size | 50-150 µm |
| Matrix | 4% agarose, crosslinked |
| Filling quantity | 1 ml pre-packed gavity flow column |
| Storage Conditions | 2-8°C |
| Shipping Conditions | RT |
FAQ
Can I get the datasheet for the Strep-Tactin®XT 4Flow® high capacity FPLC column?
Yes. Just click here to download it as a PDF File.
Is Strep-Tactin® the same as Streptavidin?
No, they are not. Strep-Tactin® is a bioengineered protein, originally based on Streptavidin. However, Strep-Tactin® cannot bind to biotin, while Streptavidin cannot bind to the Strep-tag.
Disclaimer
Strep-Tactin® and Strep-tag® are trademarks of IBA GmbH. PureCube Strep-Tactin® Agarose is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin".