PureCube Amine Activated MagBeads
Order number: 50901
Description
PureCube Amine Activated MagBeads are your best option to couple biomolecules that carry free-standing carboxy (COOH) groups. The resulting peptide bond between the Amine MagBeads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube Amine Activated MagBeads are also available as agarose resin in case you prefer this purification method, or as XL Magbeads if your downstream application involves more viscous cell media.
| Feature | |
|---|---|
| Usage | Coupling proteins to active Amine Groups |
| Specificity | Exposed carboxy (COOH) groups on biomolecule |
| Amine groupo density | 15 µmol/ml (determined by acidimetric titration) |
| Bead Ligand | Chemically active Amine groups |
| Bead size | 20-40 µm (30 µm average) |
| Filling quantity | Delivered as a 25 % suspension (20 µl suspension corresponds to 5 µl magnetic beads) |
| Storage Buffer | 20 mM sodium acetate with 20% ethanol, pH 6.5 |
| Matrix | Spherical magnetic agarose beads, cross-linked, 6% agarose |
| Short Term storage | In neutral buffer (e.g. 50 mM phosphate, pH 7.0) |
| Long Term storage | In 100 mM sodium hydrogen carbonate, 0.02% sodium azide, pH 7.5 or 20 mM sodium acetate with 20% ethanol, pH 6.5, 2-8°C |
| Shelf life | If properly stored - 2 years upon delivery, unopened |
FAQ
Can I get the datasheet for the Amine Activated MagBeads?
What can I do with these beads?
The beads carry reactive Amine groups on their surface. These can be used to bind all sorts of biomolecules if they carry free Carboxy (COOH) groups. The subsequent use of the beads depends on what biomolecule was coupled.
How does the coupling work?
How long can I store the Amine Activated Beads?
Unlike some other activated beads, amine activated beads have a long shelf life. The half-life of the amine beads is around 3 years of properly stored.