MSP Starter Kit for In Vitro Protein Synthesis (-His)

Order number: 26702

€654.00*

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Description

The MSP Starter Kit for In Vitro Protein Synthesis is ready to use for cell-free protein synthesis: it contains 8 different pre-assembled MSP-nanodiscs, which can be added directly to the expression reaction so the membrane protein inserts co-translationally into a membrane with predefined lipid composition, no detergents or post-synthesis reconstitution needed. This selection of MSP pre-assembled nanodiscs is particularly well suited for GPCRs. Two scaffold sizes (MSP1D1, MSP1E3D1) × four lipid compositions (DOPG; DOPG/Cardiolipin; POPC/POPS; POPC/POPS/Cholesterol) allow quick identification of the best-performing condition, manually or on a liquid handler. The His-tag enables fast affinity purification, and matching Scale-up vials carry the winning condition forward to μg-to-mg quantities of purified, functional membrane protein.

Datasheets

Feature
Form	preassembled MSP nanodisc
Amount	8 x 7.5 μL (500 μM)
Available origins	Human
Purity	> 90% (Determined by SDS-PAGE)
Number of amino acids	MSP1D1 - His: 217 MSPE3D1-His: 277
Molecular mass	MSP1D1-His: 25.3 kDa MSPE3D1-His: 32.6 kDa
Extinction coefficient (in H2O) ε280	MSP1D1-His: 25,440 M-1cm-1 MSPE3D1-His: 29,400 M-1cm-1
Buffer	20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
Affinity tag	N-Terminal His-tag
Stability & Storage	-80°C for several months Avoid repeated freeze-thaw cycles
Shipping	Dry ice
Hazards	Product is not classified as hazardous according to (EC) No 1272/2008 [CLP]. A Material Safety Data Sheet is provided.
Application	PPrimarily used in cell-free (in vitro) synthesis to screen and identify the best-performing nanodisc condition for a given GPCR. The His-tag then enables fast, affinity-based purification of the reconstituted receptor, supporting downstream structural studies (e.g. cryo-EM), ligand-binding and functional assays, and early-stage drug discovery screening.
Recommended usage	In Vitro Protein Synthesis 5-75 μM (0.5-7.5 μL)
Manufacturer	Cube Biotech

Citations

Video

Watch our video tutorial about the creation of MSP nanodiscs. It explains the whole process, starting with the initial solubilzation using detergents. The protein that was stabilized in this demonstration was Bacteriorhodopsin.

We also recommend our video guide to nanodiscs in general.

FAQ

Can I get the Datasheet for the MSP Starter Kit for In Vitro Protein Synthesis (-His)?

Yes. You can request it here.

I have never worked with MSP nanodiscs before. Can you tell me more about them?

Of course! We created THIS MSP NANODISC GUIDE PAGE, for this purpose. Have a look at it!

Are there other options to stabilize membrane proteins besides MSP nanodiscs?

Yes, there are. We recommend synthetic copolymer nanodiscs greatly! Have a look at THIS GUIDE PAGE to learn more.

Disclaimer

For research use only

Trademark information

The owners of trademarks marked by "®" or "TM" are identified at http://www.cube-biotech.com/information/patents. Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law.

Important licensing information

This product is covered by intellectual property (IP) rights and on completion of the sale Cube Biotech grants respective Limited Use Label Licenses to purchaser. IP rights and Limited Use Label Licenses for said technology are further described and identified at http://www.cube-biotech.com/information/patents or upon inquiry at info@cube-biotech.com or at Cube Biotech GmbH, Creative-Campus-Allee 12, D-40789 Monheim, Germany. By use of this product the purchaser accepts the terms and conditions of all applicable Limited Use Label Licenses.